• Product name

    Anti-Desmoplakin I+II antibody
    See all Desmoplakin I+II primary antibodies
  • Description

    Rabbit polyclonal to Desmoplakin I+II
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Desmoplakin I+II aa 1-100.
    (Peptide available as ab71689)

  • Positive control

    • This antibody gave a positive signal in Caco 2 whole cell lysate


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab71690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 238, 270 kDa (predicted molecular weight: 260 , 332 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • Function

    Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
  • Tissue specificity

    Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin.
  • Involvement in disease

    Keratoderma, palmoplantar, striate 2
    Cardiomyopathy, dilated, with woolly hair and keratoderma
    Arrhythmogenic right ventricular dysplasia, familial, 8
    Skin fragility-woolly hair syndrome
    Epidermolysis bullosa, lethal acantholytic
    Cardiomyopathy, dilated, with woolly hair, keratoderma, and tooth agenesis
  • Sequence similarities

    Belongs to the plakin or cytolinker family.
    Contains 17 plectin repeats.
    Contains 1 SH3 domain.
    Contains 6 spectrin repeats.
  • Domain

    Its association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.
    The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.
    The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments.
  • Post-translational

    Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.
    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377).
  • Cellular localization

    Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • 250/210 kDa paraneoplastic pemphigus antigen antibody
    • DCWHKTA antibody
    • Desmoplakin (DPI DPII) antibody
    • Desmoplakin antibody
    • Desmoplakin I antibody
    • Desmoplakin II antibody
    • DESP_HUMAN antibody
    • DP antibody
    • DP I antibody
    • DP II antibody
    • DPI antibody
    • DPII antibody
    • DSP antibody
    • KPPS2 antibody
    • PPKS 2 antibody
    • PPKS2 antibody
    see all


  • Desmoplakin I+II antibody (ab71690) at 1 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 260 , 332 kDa
    Observed band size: 238,270 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 110 kDa, 45 kDa. We are unsure as to the identity of these extra bands.

  • ICC/IF image of ab71690 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71690, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293, MCF-7 cells at 5µg/ml.


This product has been referenced in:

  • Shoaito H  et al. The Role of Peroxisome Proliferator–Activated Receptor Gamma (PPAR?) in Mono(2-ethylhexyl) Phthalate (MEHP)-Mediated Cytotrophoblast Differentiation. Environ Health Perspect 127:27003 (2019). Read more (PubMed: 30810372) »
  • Huen AC  et al. CXCL11 Expression by Keratinocytes Occurs Transiently Between Reaching Confluence and Cellular Compaction. Adv Wound Care (New Rochelle) 5:517-526 (2016). Read more (PubMed: 28078185) »
See all 10 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Mouse Cell (neuronal)
Yes - 0.2% Triton x-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Mar 21 2012

Western blot
Human Cell lysate - whole cell (human embryonic kidney cells hek293)
Loading amount
40 µg
human embryonic kidney cells hek293
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Sep 30 2011

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