Anti-Desmoplakin I+II antibody [DP2.15] (ab16434)
Key features and details
- Mouse monoclonal [DP2.15] to Desmoplakin I+II
- Suitable for: WB, ICC, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Chicken, Cow, Human
- Isotype: IgG1
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Overview
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Product name
Anti-Desmoplakin I+II antibody [DP2.15]
See all Desmoplakin I+II primary antibodies -
Description
Mouse monoclonal [DP2.15] to Desmoplakin I+II -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC, ICC/IF, IHC-Frmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Cow, Human -
Immunogen
Full length protein (Cow).
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Positive control
- Positive cell lines: Human A432 and MCF-7; Rat MH1C1; Bovine MDBK and BMGE. Stratified epithelia, simple epithelia, including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges.
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General notes
Suitable for use in the detection of primary and metastatic carcinomas.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: PBS, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
DP2.15 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration.
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ICC | (1) |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
|
IHC-Fr |
Use at an assay dependent concentration.
|
Notes |
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WB
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. -
Tissue specificity
Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin. -
Involvement in disease
Keratoderma, palmoplantar, striate 2
Cardiomyopathy, dilated, with woolly hair and keratoderma
Arrhythmogenic right ventricular dysplasia, familial, 8
Skin fragility-woolly hair syndrome
Epidermolysis bullosa, lethal acantholytic
Cardiomyopathy, dilated, with woolly hair, keratoderma, and tooth agenesis -
Sequence similarities
Belongs to the plakin or cytolinker family.
Contains 17 plectin repeats.
Contains 1 SH3 domain.
Contains 6 spectrin repeats. -
Domain
Its association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.
The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.
The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments. -
Post-translational
modificationsSer-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377). -
Cellular localization
Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 420869 Chicken
- Entrez Gene: 514360 Cow
- Entrez Gene: 1832 Human
- Entrez Gene: 109620 Mouse
- Entrez Gene: 306871 Rat
- Omim: 125647 Human
- SwissProt: P15924 Human
- SwissProt: E9Q557 Mouse
see all -
Alternative names
- 250/210 kDa paraneoplastic pemphigus antigen antibody
- DCWHKTA antibody
- Desmoplakin (DPI DPII) antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab16434 has been referenced in 31 publications.
- Fraichard C et al. Protease Inhibitor Anti-HIV, Lopinavir, Impairs Placental Endocrine Function. Int J Mol Sci 22:N/A (2021). PubMed: 33445576
- Ticiani E et al. Bisphenol S and Epidermal Growth Factor Receptor Signaling in Human Placental Cytotrophoblasts. Environ Health Perspect 129:27005 (2021). PubMed: 33605785
- Wong NR et al. Resident cardiac macrophages mediate adaptive myocardial remodeling. Immunity 54:2072-2088.e7 (2021). PubMed: 34320366
- Saxena M et al. The long non-coding RNA ET-20 mediates EMT by impairing desmosomes in breast cancer cells. J Cell Sci 134:N/A (2021). PubMed: 34633031
- Fraichard C et al. Placental production of progestins is fully effective in villous cytotrophoblasts and increases with the syncytiotrophoblast formation. Mol Cell Endocrinol 499:110586 (2020). PubMed: 31539598