Product nameAnti-Desmoplakin I+II antibody [DP2.15]
See all Desmoplakin I+II primary antibodies
DescriptionMouse monoclonal [DP2.15] to Desmoplakin I+II
Tested applicationsSuitable for: ICC, ICC/IF, WB, IHC-Frmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Chicken, Cow, Human
Full length protein (Cow).
- Positive cell lines: Human A432 and MCF-7; Rat MH1C1; Bovine MDBK and BMGE. Stratified epithelia, simple epithelia, including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges.
General notesSuitable for use in the detection of primary and metastatic carcinomas.
Storage instructionsShipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: PBS, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab16434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 331 kDa.|
FunctionMajor high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
Tissue specificityIsoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin.
Involvement in diseaseKeratoderma, palmoplantar, striate 2
Cardiomyopathy, dilated, with woolly hair and keratoderma
Arrhythmogenic right ventricular dysplasia, familial, 8
Skin fragility-woolly hair syndrome
Epidermolysis bullosa, lethal acantholytic
Cardiomyopathy, dilated, with woolly hair, keratoderma, and tooth agenesis
Sequence similaritiesBelongs to the plakin or cytolinker family.
Contains 17 plectin repeats.
Contains 1 SH3 domain.
Contains 6 spectrin repeats.
DomainIts association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.
The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.
The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments.
modificationsSer-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377).
Cellular localizationCell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity).
- Information by UniProt
- 250/210 kDa paraneoplastic pemphigus antigen antibody
- DCWHKTA antibody
- Desmoplakin (DPI DPII) antibody
ab16434 staining A431 cells by ICC/IF. Cells were methanol fixed and blocked in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/100 (PBS+10%FBS) and incubated with the sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/1500, was used as the secondary.
ab16434 staining Desmoplakin I+II in rat glial cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 5% serum) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
All lanes : Anti-Desmoplakin I+II antibody [DP2.15] (ab16434) at 1/500 dilution
Lane 1 : Control MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) treated with Desmoplakin I+II RNAi, cell lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 331 kDa
This product has been referenced in:
- Bharathan NK & Dickinson AJG Desmoplakin is required for epidermal integrity and morphogenesis in the Xenopus laevis embryo. Dev Biol 450:115-131 (2019). Read more (PubMed: 30935896) »
- Stewart RM et al. Ablation of SUN2-containing LINC complexes drives cardiac hypertrophy without interstitial fibrosis. Mol Biol Cell 30:1664-1675 (2019). Read more (PubMed: 31091167) »