Overview

  • Product name

    Anti-Desmuslin/SYN antibody
    See all Desmuslin/SYN primary antibodies
  • Description

    Rabbit polyclonal to Desmuslin/SYN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Desmuslin/SYN aa 1464-1544 (internal sequence).
    Sequence:

    MDVSNVEAIRSRTQEAGALGVSDRGSWRDADSRNDQAVGVSFKASAGEGD QAHREQGKEQAMFDKKVQLQRMVDQRSVISD


    Database link: O15061

  • Positive control

    • IHC-P: Human heart, prostate, testis and smooth muscle tissue.
  • General notes

     This product was previously labelled as Desmuslin

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells.
  • Tissue specificity

    Isoform 2 is strongly detected in adult heart, fetal skeletal muscles and fetal heart. Isoform 1 is weakly detected in fetal heart and also in fetal skeletal muscle. Isoform 1 and isoform 2 are detected in adult bladder (at protein level). The mRNA is predominantly expressed in heart and muscle with some expression in brain which may be due to tissue-specific isoforms.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    In lens, first detected at 16 weeks when expression is weakly and uniformly distributed. Subsequently, expression becomes much stronger in the epithelium of the anterior part at 25 weeks and later. In retina, weakly expressed at 15 weeks in the nerve fiber and ganglion cell layers (NFL and GCL). From 25 weeks onwards, much stronger expression is observed in the endfeet of Mueller cells, the NFL, and GCL, and much lower expression is observed in a minor subpopulation of cells in the inner cell layer (INL). At 30 and 36 weeks, expression remains in the neural retina, and subsequently becomes stronger in the NFL, GCL, and INL and is decreased in Mueller cells. At 36 weeks, also expressed at the external border of the outer nuclear layer (ONL) (at protein level).
  • Cellular localization

    Cytoplasm, cytoskeleton. Cell junction, adherens junction. There are at least two distinct SYNM subpopulations, one in which SYMN interacts with DES within the Z-lines, and another in which it interacts with both DTNA and DES at the costamere.
  • Information by UniProt
  • Database links

  • Alternative names

    • Desmuslin antibody
    • DMN antibody
    • KIAA0353 antibody
    • SYN antibody
    • SYNEM_HUMAN antibody
    • Synemin alpha antibody
    • Synemin antibody
    • Synemin beta antibody
    • Synemin, intermediate filament protein antibody
    • SYNM antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human smooth muscle tissue labeling Desmuslin/SYN with ab204369 at 1/1000 dilution.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human heart muscle tissue labeling Desmuslin/SYN with ab204369 at 1/1000 dilution.

  • Negative Control: Immunohistochemical analysis of formalin-fixed paraffin-embedded human liver labeling Desmuslin/SYN with ab204369 at 1/1000 dilution.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human colon labeling Desmuslin/SYN with ab204369 at 1/1000 dilution.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human testis labeling Desmuslin/SYN with ab204369 at 1/1000 dilution.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human prostate labeling Desmuslin/SYN with ab204369 at 1/1000 dilution.

References

ab204369 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab204369.
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