Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389)
Rabbit polyclonal Detyrosinated alpha Tubulin antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Human. Cited in 31 publication(s). Independently reviewed in 7 review(s).
- Datasheet
- References (40)
- Protocols
Overview
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Product name
Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker -
Description
Rabbit polyclonal to Detyrosinated alpha Tubulin - Microtubule Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A 10 residue synthetic peptide of the C terminal domain of human alpha Tubulin.
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General notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 10/11/2017. The following lots are still unpurified and still in stock as of 10/11/2017- GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7. Lot numbers other than GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab48389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/500. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human Detyrosinated alpha Tubulin peptide (ab191559). |
Target
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Relevance
Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of alpha and beta tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. This antibody recognizes detyrosinated alpha tubulin, a post translational modification of alpha tubulin. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Entrez Gene: 22142 Mouse
- Omim: 191110 Human
- Omim: 602528 Human
- SwissProt: P68366 Human
- SwissProt: Q13748 Human
- SwissProt: P68369 Mouse
see all -
Alternative names
- Alpha tubulin 1 antibody
- TUBA1 antibody
- TUBA2 antibody
see all
Images
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Western blot - Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389)All lanes : Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389) at 1/500 dilution
Lane 1 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Human Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-3 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Lane 4 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Detyrosinated alpha Tubulin contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed.
Protocols
References
This product has been referenced in:
- Grueb SS et al. The formin Drosophila homologue of Diaphanous2 (Diaph2) controls microtubule dynamics in colorectal cancer cells independent of its FH2-domain. Sci Rep 9:5352 (2019). Read more (PubMed: 30926831) »
- Randazzo D et al. Persistent upregulation of the ß-tubulin tubb6, linked to muscle regeneration, is a source of microtubule disorganization in dystrophic muscle. Hum Mol Genet 28:1117-1135 (2019). Read more (PubMed: 30535187) »
