Product nameAnti-Detyrosinated alpha Tubulin antibody - Microtubule Marker
DescriptionRabbit polyclonal to Detyrosinated alpha Tubulin - Microtubule Marker
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
A 10 residue synthetic peptide of the C terminal domain of human alpha Tubulin.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 10/11/2017. The following lots are still unpurified and still in stock as of 10/11/2017- GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7. Lot numbers other than GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
Concentration information loading...
PurityProtein A purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab48389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human Detyrosinated alpha Tubulin peptide (ab191559).|
RelevanceMicrotubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of alpha and beta tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. This antibody recognizes detyrosinated alpha tubulin, a post translational modification of alpha tubulin.
- Alpha tubulin 1 antibody
- TUBA1 antibody
- TUBA2 antibody
All lanes : Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389) at 1/500 dilution
Lane 1 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Human Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Lanes 1-3 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Lane 4 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Detyrosinated alpha Tubulin contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed.
This product has been referenced in:
- Grueb SS et al. The formin Drosophila homologue of Diaphanous2 (Diaph2) controls microtubule dynamics in colorectal cancer cells independent of its FH2-domain. Sci Rep 9:5352 (2019). Read more (PubMed: 30926831) »
- Randazzo D et al. Persistent upregulation of the ß-tubulin tubb6, linked to muscle regeneration, is a source of microtubule disorganization in dystrophic muscle. Hum Mol Genet 28:1117-1135 (2019). Read more (PubMed: 30535187) »