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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules Tubulin
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Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (48)

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Western blot - Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389)

    Key features and details

    • Rabbit polyclonal to Detyrosinated alpha Tubulin - Microtubule Marker
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Anti-delta II tubulin antibody (ab106658)
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    Secondary
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    View more associated products

    Overview

    • Product name

      Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker
      See all Detyrosinated alpha Tubulin primary antibodies
    • Description

      Rabbit polyclonal to Detyrosinated alpha Tubulin - Microtubule Marker
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      A 10 residue synthetic peptide of the C terminal domain of human alpha Tubulin.

    • General notes

      We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 10/11/2017. The following lots are still unpurified and still in stock as of 10/11/2017- GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7. Lot numbers other than GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microtubules
      • Tubulin

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • Human Detyrosinated alpha Tubulin peptide (ab191559)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab48389 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB (5)
    1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human Detyrosinated alpha Tubulin peptide (ab191559).
    Notes
    WB
    1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human Detyrosinated alpha Tubulin peptide (ab191559).

    Target

    • Relevance

      Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of alpha and beta tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. This antibody recognizes detyrosinated alpha tubulin, a post translational modification of alpha tubulin.
    • Cellular localization

      Cytoplasmic
    • Database links

      • Entrez Gene: 7277 Human
      • Entrez Gene: 7278 Human
      • Omim: 191110 Human
      • Omim: 602528 Human
      • SwissProt: P68366 Human
      • SwissProt: Q13748 Human
      • Unigene: 349695 Human
      • Unigene: 75318 Human
      • Alternative names

        • Alpha tubulin 1 antibody
        • TUBA1 antibody
        • TUBA2 antibody
        • TUBA3C antibody
        • TUBA4A antibody
        • Tubulin alpha 1 antibody
        • Tubulin alpha 1 chain antibody
        • Tubulin alpha 1 testis specific antibody
        • Tubulin, alpha 3c antibody
        • Tubulin, alpha 4a antibody
        see all

      Images

      • Western blot - Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389)
        Western blot - Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389)
        All lanes : Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389) at 1/500 dilution

        Lane 1 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
        Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
        Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 4 : Human Brain Tissue Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        Lanes 1-3 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
        Lane 4 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 50 kDa
        Observed band size: 51 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 28 kDa, 44 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 1 minute


        Detyrosinated alpha Tubulin contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed.

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (48)

      Publishing research using ab48389? Please let us know so that we can cite the reference in this datasheet.

      ab48389 has been referenced in 48 publications.

      • Furey C  et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393
      • Hoffmann K  et al. Stable expansion of high-grade serous ovarian cancer organoids requires a low-Wnt environment. EMBO J 39:e104013 (2020). PubMed: 32009247
      • Wen Q  et al. Myosin VIIa Supports Spermatid/Organelle Transport and Cell Adhesion During Spermatogenesis in the Rat Testis. Endocrinology 160:484-503 (2019). PubMed: 30649248
      • Liao S  et al. Molecular basis of vasohibins-mediated detyrosination and its impact on spindle function and mitosis. Cell Res 29:533-547 (2019). PubMed: 31171830
      • Grueb SS  et al. The formin Drosophila homologue of Diaphanous2 (Diaph2) controls microtubule dynamics in colorectal cancer cells independent of its FH2-domain. Sci Rep 9:5352 (2019). PubMed: 30926831
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 10 Abreviews or Q&A

      Western blot abreview for Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (Mouse Vascular smooth muscle cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      30 µg
      Specification
      Mouse Vascular smooth muscle cells
      Blocking step
      Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 31 2019

      Western blot abreview for Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (U2OS)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      35 µg
      Specification
      U2OS
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 31 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Sections of trachea- nervous structures)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
      Permeabilization
      Yes - 0.2% Triton-PBS
      Specification
      Sections of trachea- nervous structures
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 01 2019

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa cells)
      Permeabilization
      Yes - 0.2% Triton X100
      Specification
      HeLa cells
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
      Fixative
      Methanol
      Read More

      Dr. Fabienne Paumet

      Verified customer

      Submitted Apr 27 2018

      Western blot abreview for Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Cell lysate - whole cell (MTLn3 cells)
      Gel Running Conditions
      Reduced Denaturing (4-15% gradient)
      Loading amount
      140 µg
      Specification
      MTLn3 cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Neha Kaul

      Verified customer

      Submitted Sep 15 2017

      Western blot abreview for Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Pig Cell lysate - whole cell (Swine testicle cells (ST))
      Gel Running Conditions
      Reduced Denaturing (10% gel)
      Loading amount
      35 µg
      Treatment
      mock and GFP transfection
      Specification
      Swine testicle cells (ST)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
      Read More

      Mr. Robert Jansens

      Verified customer

      Submitted Mar 02 2017

      IHC - Wholemount abreview for Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      IHC - Wholemount
      Sample
      Zebrafish Embryo (whole embryos caudal fin imaged)
      Specification
      whole embryos caudal fin imaged
      Read More

      Abcam user community

      Verified customer

      Submitted May 12 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Pig Cell (swine testicle (ST))
      Permeabilization
      Yes - triton
      Specification
      swine testicle (ST)
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 11 2015

      Immunocytochemistry abreview for Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Rat Cultured Cells (Sertoli cells)
      Permeabilization
      Yes - Triton
      Specification
      Sertoli cells
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
      Fixative
      Methanol
      Read More

      Dr. Nan Li

      Verified customer

      Submitted Sep 09 2015

      Western blot abreview for Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Cell lysate - whole cell (Sertoli cells)
      Gel Running Conditions
      Reduced Denaturing (12.5% gel)
      Loading amount
      20 µg
      Specification
      Sertoli cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Dr. Nan Li

      Verified customer

      Submitted Sep 09 2015

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