Dexamethasone, anti-inflammatory glucocorticoid (ab120743)
Key features and details
- Anti-inflammatory glucocorticoid
- CAS Number: 50-02-2
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Dexamethasone, anti-inflammatory glucocorticoid -
Description
Anti-inflammatory glucocorticoid -
Biological description
Anti-inflammatory glucocorticoid (IC50 = 38 nM and Ki = 6.7 nM for the glucocorticoid receptor). Displays 7-fold higher affinity for glucocorticoid receptors than hydrocortisone. Long-term effects following oral administration. Used in the generation of liver organoids.
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Purity
> 99% -
CAS Number
50-02-2 -
Chemical structure
Properties
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Chemical name
(11β,16α)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione -
Molecular weight
392.47 -
Molecular formula
C22H29FO5 -
PubChem identifier
5743 -
Storage instructions
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C[C@@H]1C[C@H]2[C@@H]3CCC4=CC(=O)C=C[C@@]4([C@]3([C@H](C[C@@]2([C@]1(C(=O)CO)O)C)O)F)C -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Dexamethasone, anti-inflammatory glucocorticoid (ab120743)2D chemical structure image of ab120743, Dexamethasone, anti-inflammatory glucocorticoid
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Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (ab120743)ab75974 staining p57 Kip2 in HeLa cells treated with dexamethasone (ab120743), by ICC/IF. Increase in p57 Kip2 expression correlates with increased concentration of dexamethasone as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120743 (dexamethasone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab75974 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (ab120743)1e7 3T3-L1 cells were treated for 15 minutes with 1 µM dexamethasone (ab120743) and 1 µg x mL-1 insulin (ab123768) with the addition of 50 µM forskolin (ab120058), 0.5 mM IBMX (ab120840) or 0.1 mM 8-CPT-cAMP (ab120424), extracted according to the protocol. Lysates were diluted 50-fold, and tested in duplicates (+/-SD).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab120743 has been referenced in 1 publication.
- Walford HH et al. Increased ILC2s in the eosinophilic nasal polyp endotype are associated with corticosteroid responsiveness. Clin Immunol 155:126-35 (2014). PubMed: 25236785