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  1. Link

    dexamethasone-inflammatory-glucocorticoid-ab120743.pdf

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Epigenetics and Nuclear Signaling Nuclear Signaling Pathways Nuclear Receptors Corticoid
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Dexamethasone, anti-inflammatory glucocorticoid (ab120743)

  • Datasheet
  • SDS
  • COA
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Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (ab120743)
  • Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (ab120743)

Key features and details

  • Anti-inflammatory glucocorticoid
  • CAS Number: 50-02-2
  • Purity: > 99%
  • Soluble in DMSO to 100 mM
  • Form / State: Solid
  • Source: Synthetic

Overview

  • Product name

    Dexamethasone, anti-inflammatory glucocorticoid
  • Description

    Anti-inflammatory glucocorticoid
  • Biological description

    Anti-inflammatory glucocorticoid (IC50 = 38 nM and Ki = 6.7 nM for the glucocorticoid receptor). Displays 7-fold higher affinity for glucocorticoid receptors than hydrocortisone. Long-term effects following oral administration. Used in the generation of liver organoids.

  • Purity

    > 99%
  • CAS Number

    50-02-2
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    (11β,16α)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione
  • Molecular weight

    392.47
  • Molecular formula

    C22H29FO5
  • PubChem identifier

    5743
  • Storage instructions

    Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions.
  • Solubility overview

    Soluble in DMSO to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    C[C@@H]1C[C@H]2[C@@H]3CCC4=CC(=O)C=C[C@@]4([C@]3([C@H](C[C@@]2([C@]1(C(=O)CO)O)C)O)F)C
  • Source

    Synthetic

  • Research areas

    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Corticoid
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Receptors & Transporters
    • Nuclear Receptors
    • Glucocorticoid
    • Biochemicals
    • Research Area
    • Cancer
    • Nuclear Receptors
    • Glucocorticoid
    • Biochemicals
    • Research Area
    • Heart disease
    • Nuclear Receptors
    • Glucocorticoid
    • Biochemicals
    • Research Area
    • Hypertension
    • Nuclear Receptors
    • Glucocorticoid
    • Biochemicals
    • Research Area
    • Stroke
    • Nuclear Receptors
    • Glucocorticoid
    • Biochemicals
    • Pharmacology
    • Signaling
    • Stem Cells
    • Other
    • Biochemicals
    • Pharmacology
    • Signaling
    • Immunomodulators
    • Immunomodulators & Immunosuppressants

Applications

Our Abpromise guarantee covers the use of ab120743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (ab120743)
    Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (ab120743)
    ab75974 staining p57 Kip2 in HeLa cells treated with dexamethasone (ab120743), by ICC/IF. Increase in p57 Kip2 expression correlates with increased concentration of dexamethasone as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120743 (dexamethasone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab75974 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (ab120743)
    Functional Studies - Dexamethasone, anti-inflammatory glucocorticoid (ab120743)
    1e7 3T3-L1 cells were treated for 15 minutes with 1 µM dexamethasone (ab120743) and 1 µg x mL-1 insulin (ab123768) with the addition of 50 µM forskolin (ab120058), 0.5 mM IBMX (ab120840) or 0.1 mM 8-CPT-cAMP (ab120424), extracted according to the protocol. Lysates were diluted 50-fold, and tested in duplicates (+/-SD).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
    • COA
  • References (1)

    Publishing research using ab120743? Please let us know so that we can cite the reference in this datasheet.

    ab120743 has been referenced in 1 publication.

    • Walford HH  et al. Increased ILC2s in the eosinophilic nasal polyp endotype are associated with corticosteroid responsiveness. Clin Immunol 155:126-35 (2014). PubMed: 25236785

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