DFCA DA (5(6)-Carboxy-2',7'-dichlorofluorescein diacetate), Fluorogenic esterase substrate (ab145415)

Overview

  • Product name
    DFCA DA (5(6)-Carboxy-2',7'-dichlorofluorescein diacetate), Fluorogenic esterase substrate
  • Description
    Amine-reactive, fluorogenic, non-selective esterase substrate
  • Biological description

    Amine-reactive, fluorogenic, non-selective esterase substrate. Hydrolyses to DFCA (ab145439) fluorophore intracellularly. Labels intact cells. 2,7-Dichlorofluorescein (ab146332) derivative and DCFDA N-succinimidyl ester (ab145286) precursor.

  • Purity
    > 85%
  • CAS Number
    127770-45-0
  • Chemical structure
    Chemical Structure

Properties

  • Excitation
    495nm
  • Emission
    529nm
  • Chemical name
    5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate
  • Molecular weight
    529.28
  • Molecular formula
    C25H14Cl2O9
  • PubChem identifier
    16211578
  • Storage instructions
    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview
    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES
    CC(=O)OC1=C(C=C2C(=C1)OC3=CC(=C(C=C3C24C5=C(C=C(C=C5)C(=O)O)C(=O)O4)Cl)OC(=O)C)Cl.CC(=O)OC1=C(C=C2C(=C1)OC3=CC(=C(C=C3C24C5=C(C=CC(=C5)C(=O)O)C(=O)O4)Cl)OC(=O)C)Cl
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:
  • Janda E  et al. Parkinsonian toxin-induced oxidative stress inhibits basal autophagy in astrocytes via NQO2/Quinone Oxidoreductase 2: implications for neuroprotection. Autophagy :0 (2015). Read more (PubMed: 26046590) »
  • Guo C  et al. Alpha-naphthylisothiocyanate modulates hepatobiliary transporters in sandwich-cultured rat hepatocytes. Toxicol Lett 224:93-100 (2014). Read more (PubMed: 24120425) »
  • Zamek-Gliszczynski MJ  et al. Pharmacokinetics of 5 (and 6)-carboxy-2',7'-dichlorofluorescein and its diacetate promoiety in the liver. J Pharmacol Exp Ther 304:801-9 (2003). Read more (PubMed: 12538836) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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