For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    dffbcad-antibody-2263c6a-ab50744.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Nucleus DNAses
Share by email

Anti-DFFB/CAD antibody [2263C6a] (ab50744)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-DFFB/CAD antibody [2263C6a] (ab50744)

    Key features and details

    • Mouse monoclonal [2263C6a] to DFFB/CAD
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human DFFB/CAD protein (ab158274)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-DFFB/CAD antibody [2263C6a]
      See all DFFB/CAD primary antibodies
    • Description

      Mouse monoclonal [2263C6a] to DFFB/CAD
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      WB
      Recombinant fragment
      See all applications and species data
    • Immunogen

      Recombinant fragment corresponding to Human DFFB/CAD. Recombinant human DFFB/CAD fragment carying 50-200 aa

    • Positive control

      • Recombinant human DFFB/CAD
    • General notes

       

      Product previously known as Anti-DFFB antibody [2263C6a]

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 1% BSA, PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      ab50744 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
    • Clonality

      Monoclonal
    • Clone number

      2263C6a
    • Isotype

      IgG1
    • Research areas

      • Cell Biology
      • Apoptosis
      • Nucleus
      • DNAses
      • Cell Biology
      • Apoptosis
      • Nucleus
      • Chromatin Condensation
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Apoptosis
      • Nuclear
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Nucleus
      • DNAses
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human DFFB/CAD protein (ab158274)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab50744 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Recombinant fragment
    Application Abreviews Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
    Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.

    Target

    • Function

      Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
    • Sequence similarities

      Contains 1 CIDE-N domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession O76075 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • CAD antibody
      • Caspase activated deoxyribonuclease antibody
      • Caspase activated DNase antibody
      • Caspase activated nuclease antibody
      • Caspase-activated deoxyribonuclease antibody
      • Caspase-activated DNase antibody
      • Caspase-activated nuclease antibody
      • CPAN antibody
      • Deoxyribonuclease III, caspase activated antibody
      • DFF 40 antibody
      • DFF-40 antibody
      • DFF2 antibody
      • DFF40 antibody
      • Dffb antibody
      • DFFB_HUMAN antibody
      • Didff antibody
      • DNA fragmentation factor 40 kDa subunit antibody
      • DNA fragmentation factor subunit beta antibody
      • DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase) antibody
      • DNA fragmentation factor, 40 kD beta subunit antibody
      • DNA fragmentation factor, 40 kD, beta polypeptide antibody
      • DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase) antibody
      • OTTHUMP00000003633 antibody
      see all

    Images

    • Western blot - Anti-DFFB/CAD antibody [2263C6a] (ab50744)
      Western blot - Anti-DFFB/CAD antibody [2263C6a] (ab50744)
      Anti-DFFB/CAD antibody [2263C6a] (ab50744) + immunized recombinant protein

      Predicted band size: 32 kDa
      Observed band size: 34 kDa
      why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab50744? Please let us know so that we can cite the reference in this datasheet.

    ab50744 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab50744.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.