Anti-DFFB/CAD antibody [2263C6a] (ab50744)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-DFFB/CAD antibody [2263C6a]
See all DFFB/CAD primary antibodies -
Description
Mouse monoclonal [2263C6a] to DFFB/CAD -
Host species
Mouse -
Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human DFFB/CAD. Recombinant human DFFB/CAD fragment carying 50-200 aa
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Positive control
- Recombinant human DFFB/CAD
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General notes
Product previously known as Anti-DFFB antibody [2263C6a]
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab50744 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
2263C6a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab50744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. Predicted molecular weight: 32 kDa. | |
| Dot blot | Use at an assay dependent dilution. |
Target
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Function
Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. -
Sequence similarities
Contains 1 CIDE-N domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1677 Human
- Omim: 601883 Human
- SwissProt: O76075 Human
- Unigene: 133089 Human
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Alternative names
- CAD antibody
- Caspase activated deoxyribonuclease antibody
- Caspase activated DNase antibody
see all
Images
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![Western blot - Anti-DFFB/CAD antibody [2263C6a] (ab50744)](https://www.abcam.com/ps/products/50/ab50744/Images/ab50744-62421-anti-dffb-cad-antibody-2263c6a-western-blot.jpg)
Western blot - Anti-DFFB/CAD antibody [2263C6a] (ab50744)Anti-DFFB/CAD antibody [2263C6a] (ab50744) + immunized recombinant protein
Predicted band size: 32 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
ab50744 has not yet been referenced specifically in any publications.
