Product nameAnti-DFFB/CAD antibody
See all DFFB/CAD primary antibodies
DescriptionRabbit polyclonal to DFFB/CAD
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human DFFB/CAD aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: Jurkat; Raji; K562; HeLa; HeLa Nuclear.
Product previously known as Anti-DFFB antibody
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab69438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionNuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Sequence similaritiesContains 1 CIDE-N domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- CAD antibody
- Caspase activated deoxyribonuclease antibody
- Caspase activated DNase antibody
All lanes : Anti-DFFB/CAD antibody (ab69438) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 15 kDa (possible isoform), 30 kDa (possible isoform), 75 kDa, 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3 and 4) of DNA fragmentation factor subunit beta (DFFB/CAD). The predicted molecular weights of isoforms (1,2,3 and 4) are 39 kDa, 30 kDa, 12 kDa and 11 kDa respectively.
ICC/IF image of ab69438 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69438, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, and MCF-7 cells at 5µg/ml.
ab69438 has not yet been referenced specifically in any publications.