• Product name

  • Description

    Rabbit polyclonal to DFFB/CAD
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse DFFB/CAD aa 147-164.
    Database link: O76075
    (Peptide available as ab8438)

  • Positive control

    • K562 or Jurkat whole cell lysate A 40 kDa band can be detected. IHC-P: FFPE human ovary tissue sections.
  • General notes

    Product previously known as Anti-DFFB antibody.




Our Abpromise guarantee covers the use of ab8406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa.


  • Function

    Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
  • Sequence similarities

    Contains 1 CIDE-N domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAD antibody
    • Caspase activated deoxyribonuclease antibody
    • Caspase activated DNase antibody
    • Caspase activated nuclease antibody
    • Caspase-activated deoxyribonuclease antibody
    • Caspase-activated DNase antibody
    • Caspase-activated nuclease antibody
    • CPAN antibody
    • Deoxyribonuclease III, caspase activated antibody
    • DFF 40 antibody
    • DFF-40 antibody
    • DFF2 antibody
    • DFF40 antibody
    • Dffb antibody
    • DFFB_HUMAN antibody
    • Didff antibody
    • DNA fragmentation factor 40 kDa subunit antibody
    • DNA fragmentation factor subunit beta antibody
    • DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase) antibody
    • DNA fragmentation factor, 40 kD beta subunit antibody
    • DNA fragmentation factor, 40 kD, beta polypeptide antibody
    • DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase) antibody
    • OTTHUMP00000003633 antibody
    see all


  • All lanes : Anti-DFFB/CAD antibody (ab8406) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : K562 whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : Raji whole cell lysate
  • IHC image of DDFB/CAD staining in human ovary formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8406, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab8406 at 20μg/ml staining DFFB/CAD in K562 cells by ICC/IF.

  • ab8406 at 5µg/ml staining DFFB/CAD in Jurkat cells by ICC/IF


This product has been referenced in:

  • Yadav VK  et al. Prostaglandin F2alpha-mediated activation of apoptotic signaling cascades in the corpus luteum during apoptosis: involvement of caspase-activated DNase. J Biol Chem 280:10357-67 (2005). Read more (PubMed: 15623530) »
See 1 Publication for this product

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