Key features and details
- Rabbit polyclonal to DFNA5/GSDME
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DFNA5/GSDME antibody
See all DFNA5/GSDME primary antibodies
DescriptionRabbit polyclonal to DFNA5/GSDME
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human DFNA5/GSDME (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O60443
- IHC-P: Human colon tissue. WB: HeLa whole cell lysate (ab150035).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 54 kDa.|
Tissue specificityExpressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.
Involvement in diseaseDefects in DFNA5 are the cause of deafness autosomal dominant type 5 (DFNA5) [MIM:600994]. DFNA5 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similaritiesBelongs to the gasdermin family.
- Information by UniProt
- 2310037D07Rik antibody
- 4932441K13Rik antibody
- Deafness, autosomal dominant 5 antibody
Anti-DFNA5/GSDME antibody (ab230482) at 1/500 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Developed using the ECL technique.
Predicted band size: 54 kDa
Formalin-fixed, paraffin-embedded human colon tissue stained for DFNA5/GSDME with ab230482 at a 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230482 has not yet been referenced specifically in any publications.