Key features and details
- Rabbit polyclonal to DFNA5/GSDME
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DFNA5/GSDME antibody
See all DFNA5/GSDME primary antibodies
DescriptionRabbit polyclonal to DFNA5/GSDME
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human placenta tissue. WB: RT4 and U-251 MG cell lysate. ICC/IF: A431 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityExpressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.
Involvement in diseaseDefects in DFNA5 are the cause of deafness autosomal dominant type 5 (DFNA5) [MIM:600994]. DFNA5 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similaritiesBelongs to the gasdermin family.
- Information by UniProt
- 2310037D07Rik antibody
- 4932441K13Rik antibody
- Deafness, autosomal dominant 5 antibody
PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling DFNA5/GSDME using ab244323 at 4 µg/ml (green) in ICC/IF.
Formalin-fixed, paraffin-embedded human placenta tissue stained for DFNA5/GSDME with ab244323 at a 1/20 dilution in immunohistochemical analysis.
All lanes : Anti-DFNA5/GSDME antibody (ab244323) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244323 has not yet been referenced specifically in any publications.