Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)

Overview

  • Product name

    Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal
    See all DFNA5/GSDME primary antibodies
  • Description

    Rabbit monoclonal [EPR19859-60] to DFNA5/GSDME - C-terminal
  • Host species

    Rabbit
  • Specificity

    Expression levels of the target protein vary with sample type and some optimisation may be required.

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human DFNA5/GSDME aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: O60443

  • Positive control

    • WB: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate. HEK-293 transfected with a myc tagged, DFNA5/GSDME (C-terminus) expression vector, whole cell lysate. Human fetal brain lysate. IP: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19859-60
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 36, 55 kDa (predicted molecular weight: 54 kDa).
IP 1/40.

Target

  • Tissue specificity

    Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.
  • Involvement in disease

    Defects in DFNA5 are the cause of deafness autosomal dominant type 5 (DFNA5) [MIM:600994]. DFNA5 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similarities

    Belongs to the gasdermin family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2310037D07Rik antibody
    • 4932441K13Rik antibody
    • Deafness, autosomal dominant 5 antibody
    • Deafness, autosomal dominant 5 protein antibody
    • DFNA5 antibody
    • DFNA5 gene antibody
    • DFNA5_HUMAN antibody
    • Dfna5h antibody
    • EG14210 antibody
    • Fin15 antibody
    • ICERE 1 antibody
    • ICERE-1 antibody
    • Inversely correlated with estrogen receptor expression 1 antibody
    • Non-syndromic hearing impairment protein 5 antibody
    • Nonsyndromic hearing impairment protein antibody
    see all

Images

  • All lanes : Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/5000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
    Lane 2 : DFNA5/GSDME knockout SH-SY5Y, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa


    Exposure time: 30 seconds


    Blocking/Dilution: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.

  • All lanes : Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/1000 dilution

    Lane 1 : HEK-293 (human embryonic kidney epithelial cell) transfected with a control vector containing DDDDK tag, whole cell lysate
    Lane 2 : HEK-293 transfected with a DDDDK tagged, DFNA5/GSDME (N-terminus) expression vector, whole cell lysate
    Lane 3 : HEK-293 transfected with a myc tagged, DFNA5/GSDME (C-terminus) expression vector, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa


    Exposure time: 5 seconds


    Blocking/Dilution: 5% NFDM/TBST .

    This antibody is specific to the C-terminus of DFNA5/GSDME.

    The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.

  • Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/1000 dilution + human fetal brain at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa


    Exposure time: 3 minutes


    Blocking/Dilution: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 28459430).

  • DFNA5/GSDME was immunoprecipitated from 0.35 mg of SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab221843 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab221843 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: SH-SY5Y whole cell lysate 10ug (Input).
    Lane 2: ab221843 IP in SH-SY5Y whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221843 in SH-SY5Y whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Observed MW 55 kDa.
    Exposure time: 1 second.

References

This product has been referenced in:

See 1 Publication for this product

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