Anti-DGAT1 antibody (ab122924)

Reviews (1)Q&A (1)References (1)

Overview

  • Product name

    Anti-DGAT1 antibody
    See all DGAT1 primary antibodies

  • Description

    Goat polyclonal to DGAT1

  • Host species

    Goat

  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Sheep, Cow, Dog

  • Immunogen

    Synthetic peptide:

    C-QNSMKPFKDMDYS

    with a Cysteine residue linker, corresponding to internal sequence amino acids 305-317 of Human DGAT1 (NP_036211.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human Colon (ganglion) tissue; Rat duodenum lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab122924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P Use a concentration of 3.75 µg/ml.
ELISA 1/64000.

Target

  • Function

    Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly.

  • Pathway

    Lipid metabolism; glycerolipid metabolism.

  • Sequence similarities

    Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ACAT related gene product 1 antibody
    • ACAT-related gene product 1 antibody
    • Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
    • Acyl-CoA retinol O-fatty-acyltransferase antibody
    • Acyl-CoA:diacylglycerol acyltransferase antibody
    • ARAT antibody
    • ARGP1 antibody
    • C75990 antibody
    • D15Ertd23e antibody
    • Dgat antibody
    • DGAT1 antibody
    • DGAT1_HUMAN antibody
    • Diacylglycerol O acyltransferase 1 antibody
    • Diacylglycerol O-acyltransferase 1 antibody
    • DIAR7 antibody
    • Diglyceride acyltransferase antibody
    • EC 2.3.1.20 antibody
    • hCG_24006 antibody
    • MGC139064 antibody
    • Retinol O fatty acyltransferase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT1 antibody (ab122924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT1 antibody (ab122924)
    ab122924, at 3.75 µg/ml, staining DGAT1 in Formalin-fixed, Paraffin-embedded Human Colon (ganglion) tissue by Immunohistochemistry.
  • Western blot - Anti-DGAT1 antibody (ab122924)
    Western blot - Anti-DGAT1 antibody (ab122924)
    Anti-DGAT1 antibody (ab122924) at 0.3 µg/ml + Rat duodenum lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa

References

This product has been referenced in:

  • Yang W  et al. Betaine attenuates chronic alcohol-induced fatty liver by broadly regulating hepatic lipid metabolism. Mol Med Rep 16:5225-5234 (2017). Read more (PubMed: 28849079) »
See 1 Publication for this product

Publishing research using ab122924? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-DGAT1 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Horse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Denaturing (12 %)
Loading amount
60 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted May 23 2017

Question

Thank you for your fast reply. Here below you can find the full length sequence of the fly DGAT1 :

MTTNKDPQDKEPGKAEQPTKNSGSSGVGIMKRLRRSASATEHNLSSLRNRKSTQNLF
DQHGNPIDLRQYRKVLDKDENGNGTNGSEKKLRYRRTQSVTRAEEISNKEEKQRRAQ
PGRPIHRPRDSLFSWSSGFTNFSGLVNWGFLLLCIGGLRLGLENLLKYGIRINPLDW
FFFISGHNEGEGHNALILSIYSLVHISLCLAVEKGLAMEIIAEGLGLFIQIVNIVVL
VCLPVVTIHLKGHAFSLMGASTVCFFYSVLFLKLWSYVQTNMWCRQTYYQKNPRERR
PSITLAELKKGVLNGGEEDEDVSKLVQYPDNLTYKDLLYFLCAPTLCYELNFPRTSR
VRKRFLLKRLLEVVIGVNVVMALFQQWIIPSVRNSLIPFSNMDVALATERLLKLALP
NHLCWLCFFYLMFHSFLNAVGELLNFADRNFYCDWWNANNIDTFWRTWNMPVHRWCV
RHLYIPVVQMGYSSRQASTIVFLFSAVFHEYLVSVPLQIYKIWAFMGMMGQIPLSAI
SKSIEKKLGPRMGNIIVWASIILGQPLCIMAYYHDYVVQHFKNSLNGTDYSS

We would be really interested if one of your 3 other references could work for fruit flies.
Best regards,

Read More
Answer

Thank you for your reply and the sequence.

Unfortunately, after investigation I confirm that none of the 4 anti-DGAT1 antibodies is likely to work in Drosophila. The immunogens used to raise ab59034, ab122924 and ab100982 do not show more than 46% homology with the fly DGAT1.
I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The link is http://www.biocompare.com.

We are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services visit http://www.epitomics.com/services/ or email mailto:service@epitomics.com.
Alternatively, please contact Li Li at Epitomics: mailto:li.li@epitomics.com

I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

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