Anti-DGAT1 antibody (ab122924)
Key features and details
- Goat polyclonal to DGAT1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DGAT1 antibody
See all DGAT1 primary antibodies -
Description
Goat polyclonal to DGAT1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Sheep, Cow, Dog
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Immunogen
Synthetic peptide:
C-QNSMKPFKDMDYS
with a Cysteine residue linker, corresponding to internal sequence amino acids 305-317 of Human DGAT1 (NP_036211.2). -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa.
|
| IHC-P |
Use a concentration of 3.75 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa. |
|
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
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Function
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. -
Pathway
Lipid metabolism; glycerolipid metabolism. -
Sequence similarities
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 282609 Cow
- Entrez Gene: 482093 Dog
- Entrez Gene: 8694 Human
- Entrez Gene: 13350 Mouse
- Entrez Gene: 84497 Rat
- Omim: 604900 Human
- SwissProt: Q8MK44 Cow
- SwissProt: O75907 Human
see all -
Alternative names
- ACAT related gene product 1 antibody
- ACAT-related gene product 1 antibody
- Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT1 antibody (ab122924)ab122924, at 3.75 µg/ml, staining DGAT1 in Formalin-fixed, Paraffin-embedded Human Colon (ganglion) tissue by Immunohistochemistry.
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Western blot - Anti-DGAT1 antibody (ab122924)Anti-DGAT1 antibody (ab122924) at 0.3 µg/ml + Rat duodenum lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 55 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab122924 has been referenced in 1 publication.
- Yang W et al. Betaine attenuates chronic alcohol-induced fatty liver by broadly regulating hepatic lipid metabolism. Mol Med Rep 16:5225-5234 (2017). PubMed: 28849079