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    dgat1-antibody-ab122924.pdf

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Signal Transduction Signaling Pathway Lipid Signaling Other
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Anti-DGAT1 antibody (ab122924)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT1 antibody (ab122924)
  • Western blot - Anti-DGAT1 antibody (ab122924)

Key features and details

  • Goat polyclonal to DGAT1
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

View more associated products

Overview

  • Product name

    Anti-DGAT1 antibody
    See all DGAT1 primary antibodies
  • Description

    Goat polyclonal to DGAT1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Sheep, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-QNSMKPFKDMDYS

    with a Cysteine residue linker, corresponding to internal sequence amino acids 305-317 of Human DGAT1 (NP_036211.2).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Other
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab122924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P
Use a concentration of 3.75 µg/ml.
Notes
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P
Use a concentration of 3.75 µg/ml.

Target

  • Function

    Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly.
  • Pathway

    Lipid metabolism; glycerolipid metabolism.
  • Sequence similarities

    Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession O75907 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282609 Cow
    • Entrez Gene: 482093 Dog
    • Entrez Gene: 8694 Human
    • Entrez Gene: 13350 Mouse
    • Entrez Gene: 84497 Rat
    • Omim: 604900 Human
    • SwissProt: Q8MK44 Cow
    • SwissProt: O75907 Human
    • SwissProt: Q9Z2A7 Mouse
    • SwissProt: Q9ERM3 Rat
    • Unigene: 521954 Human
    • Unigene: 22633 Mouse
    • Unigene: 252 Rat
    see all
  • Alternative names

    • ACAT related gene product 1 antibody
    • ACAT-related gene product 1 antibody
    • Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
    • Acyl-CoA retinol O-fatty-acyltransferase antibody
    • Acyl-CoA:diacylglycerol acyltransferase antibody
    • ARAT antibody
    • ARGP1 antibody
    • C75990 antibody
    • D15Ertd23e antibody
    • Dgat antibody
    • DGAT1 antibody
    • DGAT1_HUMAN antibody
    • Diacylglycerol O acyltransferase 1 antibody
    • Diacylglycerol O-acyltransferase 1 antibody
    • DIAR7 antibody
    • Diglyceride acyltransferase antibody
    • EC 2.3.1.20 antibody
    • hCG_24006 antibody
    • MGC139064 antibody
    • Retinol O fatty acyltransferase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT1 antibody (ab122924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT1 antibody (ab122924)
    ab122924, at 3.75 µg/ml, staining DGAT1 in Formalin-fixed, Paraffin-embedded Human Colon (ganglion) tissue by Immunohistochemistry.
  • Western blot - Anti-DGAT1 antibody (ab122924)
    Western blot - Anti-DGAT1 antibody (ab122924)
    Anti-DGAT1 antibody (ab122924) at 0.3 µg/ml + Rat duodenum lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab122924? Please let us know so that we can cite the reference in this datasheet.

ab122924 has been referenced in 1 publication.

  • Yang W  et al. Betaine attenuates chronic alcohol-induced fatty liver by broadly regulating hepatic lipid metabolism. Mol Med Rep 16:5225-5234 (2017). PubMed: 28849079

Customer reviews and Q&As

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Western blot abreview for Anti-DGAT1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Horse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Denaturing (12 %)
Loading amount
60 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted May 23 2017

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