Overview

  • Product name
  • Description
    Goat polyclonal to DGAT1
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human DGAT1 aa 67-79 (internal sequence).
    Sequence:

    RCHRLQDSLFSSD


    Database link: NP_036211.2

  • Positive control
    • Mouse Duodenum lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab177236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50, 55 kDa (predicted molecular weight: 55 kDa).

Target

  • Function
    Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly.
  • Pathway
    Lipid metabolism; glycerolipid metabolism.
  • Sequence similarities
    Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAT related gene product 1 antibody
    • ACAT-related gene product 1 antibody
    • Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
    • Acyl-CoA retinol O-fatty-acyltransferase antibody
    • Acyl-CoA:diacylglycerol acyltransferase antibody
    • ARAT antibody
    • ARGP1 antibody
    • C75990 antibody
    • D15Ertd23e antibody
    • Dgat antibody
    • DGAT1 antibody
    • DGAT1_HUMAN antibody
    • Diacylglycerol O acyltransferase 1 antibody
    • Diacylglycerol O-acyltransferase 1 antibody
    • DIAR7 antibody
    • Diglyceride acyltransferase antibody
    • EC 2.3.1.20 antibody
    • hCG_24006 antibody
    • MGC139064 antibody
    • Retinol O fatty acyltransferase antibody
    see all

Images

  • Anti-DGAT1 antibody (ab177236) at 0.5 µg/ml + Mouse Duodenum lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 50,55 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

ab177236 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Duodenum)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
40 µg
Specification
Duodenum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

Submitted May 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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