The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50, 55 kDa (predicted molecular weight: 55 kDa).
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly.
Lipid metabolism; glycerolipid metabolism.
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.