• Product name
  • Description
    Goat polyclonal to DGAT1
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Goat, Cow, Dog, Pig, African green monkey
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 305-317 of Human DGAT1

  • Positive control
    • Human Duodenum lysate.



Our Abpromise guarantee covers the use of ab59034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    Peptide ELISA: antibody detection limit dilution 1:16,000.

    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly.
    • Pathway
      Lipid metabolism; glycerolipid metabolism.
    • Sequence similarities
      Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAT related gene product 1 antibody
      • ACAT-related gene product 1 antibody
      • Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
      • Acyl-CoA retinol O-fatty-acyltransferase antibody
      • Acyl-CoA:diacylglycerol acyltransferase antibody
      • ARAT antibody
      • ARGP1 antibody
      • C75990 antibody
      • D15Ertd23e antibody
      • Dgat antibody
      • DGAT1 antibody
      • DGAT1_HUMAN antibody
      • Diacylglycerol O acyltransferase 1 antibody
      • Diacylglycerol O-acyltransferase 1 antibody
      • DIAR7 antibody
      • Diglyceride acyltransferase antibody
      • EC antibody
      • hCG_24006 antibody
      • MGC139064 antibody
      • Retinol O fatty acyltransferase antibody
      see all


    • Anti-DGAT1 antibody (ab59034) at 0.5 µg/ml + Human Duodenum lysate (35µg protein in RIPA buffer)

      Predicted band size: 55 kDa
      Observed band size: 50 kDa (why is the actual band size different from the predicted?)

      Primary incubation was 1 hour. Detected by chemiluminescence.


    This product has been referenced in:
    • Soayfane Z  et al. Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane. Nutr Metab (Lond) 13:48 (2016). Read more (PubMed: 27478484) »
    • Kaylor JJ  et al. Diacylglycerol O-acyltransferase type-1 synthesizes retinyl esters in the retina and retinal pigment epithelium. PLoS One 10:e0125921 (2015). WB . Read more (PubMed: 25974161) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Thank you for your reply and the sequence.

    Unfortunately, after investigation I confirm that none of the 4 anti-DGAT1 antibodies is likely to work in Drosophila. The immunogens used to raise ab59034, ab122924 and ab100982 do not show more than...

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    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passin...

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    Thank you for contacting Abcam regarding ab59034.

    I am sorry that this antibody is not working for you in WB. I have reviewed the protocol information and data you sent and I agree that this product is not performing as stated on the data...

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    Thank you for contacting us. I have just been informed that the immunogen peptide of the anti-DGAT1 ab59034 is now available. The catalog reference number is ab118966 and the link to the datasheet is www.abcam.com/ab118966. I hope this information is h...

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