Overview

  • Product name
  • Description
    Goat polyclonal to DGAT1
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Goat, Cow, Dog, Pig, African green monkey
  • Immunogen

    Synthetic peptide:

    C-QNSMKPFKDMDYS

    , corresponding to internal sequence amino acids 305-317 of Human DGAT1

  • Positive control
    • Human Duodenum lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab59034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Peptide ELISA: antibody detection limit dilution 1:16,000.

    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly.
    • Pathway
      Lipid metabolism; glycerolipid metabolism.
    • Sequence similarities
      Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAT related gene product 1 antibody
      • ACAT-related gene product 1 antibody
      • Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
      • Acyl-CoA retinol O-fatty-acyltransferase antibody
      • Acyl-CoA:diacylglycerol acyltransferase antibody
      • ARAT antibody
      • ARGP1 antibody
      • C75990 antibody
      • D15Ertd23e antibody
      • Dgat antibody
      • DGAT1 antibody
      • DGAT1_HUMAN antibody
      • Diacylglycerol O acyltransferase 1 antibody
      • Diacylglycerol O-acyltransferase 1 antibody
      • DIAR7 antibody
      • Diglyceride acyltransferase antibody
      • EC 2.3.1.20 antibody
      • hCG_24006 antibody
      • MGC139064 antibody
      • Retinol O fatty acyltransferase antibody
      see all

    Images

    • Anti-DGAT1 antibody (ab59034) at 0.5 µg/ml + Human Duodenum lysate (35µg protein in RIPA buffer)

      Predicted band size: 55 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?



      Primary incubation was 1 hour. Detected by chemiluminescence.

    References

    This product has been referenced in:
    • Soayfane Z  et al. Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane. Nutr Metab (Lond) 13:48 (2016). Read more (PubMed: 27478484) »
    • Kaylor JJ  et al. Diacylglycerol O-acyltransferase type-1 synthesizes retinyl esters in the retina and retinal pigment epithelium. PLoS One 10:e0125921 (2015). WB . Read more (PubMed: 25974161) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for your reply and the sequence.

    Unfortunately, after investigation I confirm that none of the 4 anti-DGAT1 antibodies is likely to work in Drosophila. The immunogens used to raise ab59034, ab122924 and ab100982 do not show more than 46% homology with the fly DGAT1.
    I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The link is http://www.biocompare.com.

    We are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services visit http://www.epitomics.com/services/ or email mailto:service@epitomics.com.
    Alternatively, please contact Li Li at Epitomics: mailto:li.li@epitomics.com

    I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

    Read More

    Answer

    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Answer

    Thank you for contacting Abcam regarding ab59034.


    I am sorry that this antibody is not working for you in WB. I have reviewed the protocol information and data you sent and I agree that this product is not performing as stated on the datasheet. I am happy to offer a replacement or credit, per our Abpromise guarantee. Please let me knowwhich you would prefer.


    I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Answer

    Thank you for contacting us. I have just been informed that the immunogen peptide of the anti-DGAT1 ab59034 is now available. The catalog reference number is ab118966 and the link to the datasheet is www.abcam.com/ab118966. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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