Anti-DGAT1 antibody (ab59034)
Key features and details
- Goat polyclonal to DGAT1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DGAT1 antibody
See all DGAT1 primary antibodies -
Description
Goat polyclonal to DGAT1 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Goat, Cow, Dog, Pig, African green monkey -
Immunogen
Synthetic peptide:
C-QNSMKPFKDMDYS
, corresponding to internal sequence amino acids 305-317 of Human DGAT1 -
Positive control
- WB: Human duodenum and mouse liver tissue lysates. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 3.75 µg/ml.
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WB | (2) |
Use a concentration of 0.01 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
A 1 hour primary incubation is recommended for this product. |
ICC/IF |
Use a concentration of 10 µg/ml.
|
Notes |
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IHC-P
Use a concentration of 3.75 µg/ml. |
WB
Use a concentration of 0.01 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). A 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. -
Pathway
Lipid metabolism; glycerolipid metabolism. -
Sequence similarities
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 282609 Cow
- Entrez Gene: 482093 Dog
- Entrez Gene: 8694 Human
- Entrez Gene: 13350 Mouse
- Entrez Gene: 397118 Pig
- Entrez Gene: 84497 Rat
- Omim: 604900 Human
- SwissProt: Q8MK44 Cow
see all -
Alternative names
- ACAT related gene product 1 antibody
- ACAT-related gene product 1 antibody
- Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
see all
Images
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immunofluorescence analysis of A431 cells labelling DGAT1 with ab59034 at 10 µg/mL or dilution]. Cells were permeabilized with 0.15% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 2 µg/mL was used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG 10 µg/mL. Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm is seen.
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Anti-DGAT1 antibody (ab59034) at 0.03 µg/ml + Mouse liver lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 55 kDaPrimary incubation: 1 hour at room temperature.
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Anti-DGAT1 antibody (ab59034) at 0.5 µg/ml + Human Duodenum lysate (35µg protein in RIPA buffer)
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (5)
ab59034 has been referenced in 5 publications.
- Lv Y et al. Metabolic Transition of Milk Triacylglycerol Synthesis in Response to Varying Levels of Three 18-Carbon Fatty Acids in Porcine Mammary Epithelial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33525494
- Howie D et al. A Novel Role for Triglyceride Metabolism in Foxp3 Expression. Front Immunol 10:1860 (2019). PubMed: 31456800
- Soayfane Z et al. Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane. Nutr Metab (Lond) 13:48 (2016). PubMed: 27478484
- Kaylor JJ et al. Diacylglycerol O-acyltransferase type-1 synthesizes retinyl esters in the retina and retinal pigment epithelium. PLoS One 10:e0125921 (2015). WB . PubMed: 25974161
- Buers I et al. TIP47, a lipid cargo protein involved in macrophage triglyceride metabolism. Arterioscler Thromb Vasc Biol 29:767-73 (2009). PubMed: 19286631