Recombinant
RabMAb

Recombinant Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free (ab240250)

Overview

  • Product name

    Anti-DGAT1 antibody [EPR13430-4] - BSA and Azide free
    See all DGAT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13430-4] to DGAT1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human DGAT1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O75907

  • Positive control

    • WB: HeLa cell lysate, HEK-293 and Jurkat whole cell lysate prepared in 1%SDS Hot lysis method Human small intestine lysates IP: Human fetal kidney lysate. ICC/IF: MCF7, HeLa cells. FC: HeLa cells.
  • General notes

    Ab240250 is the carrier-free version of ab181180. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240250 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly.
  • Pathway

    Lipid metabolism; glycerolipid metabolism.
  • Sequence similarities

    Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACAT related gene product 1 antibody
    • ACAT-related gene product 1 antibody
    • Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody
    • Acyl-CoA retinol O-fatty-acyltransferase antibody
    • Acyl-CoA:diacylglycerol acyltransferase antibody
    • ARAT antibody
    • ARGP1 antibody
    • C75990 antibody
    • D15Ertd23e antibody
    • Dgat antibody
    • DGAT1 antibody
    • DGAT1_HUMAN antibody
    • Diacylglycerol O acyltransferase 1 antibody
    • Diacylglycerol O-acyltransferase 1 antibody
    • DIAR7 antibody
    • Diglyceride acyltransferase antibody
    • EC 2.3.1.20 antibody
    • hCG_24006 antibody
    • MGC139064 antibody
    • Retinol O fatty acyltransferase antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1:100 dilution (4.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180)

  • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling DGAT1 with Purified ab181180 at 1:400 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180)

  • ab181180 (purified) at 1:30 dilution (2µg) immunoprecipitating DGAT1 in Human fetal kidney lysate.
    Lane 1 (input): Human fetal kidney lysate 10µg
    Lane 2 (+): ab181180 & Human fetal kidney lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181180 in Human fetal kidney lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180)

  • Western blot analysis on immunoprecipitation pellet from Human fetal kidney lysate (lane 1) or negative control (lane 2), labeling DGAT1 immunoprecipitated using ab181180 at 1/1500 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180).

  • Flow cytometry analysis of DGAT1 expression in HeLa cells using ab181180 at 1/70 dilution (red) and a rabbit IgG as negative control (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180).

  • Immunofluorescence analysis of acetone-fixed HeLa cells, staining DGAT1 (green) with ab181180 at 1/100 dilution. Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181180).

References

ab240250 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab240250.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up