Anti-DGAT2 antibody (ab59493)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-DGAT2 antibody
See all DGAT2 primary antibodies -
Description
Goat polyclonal to DGAT2 -
Host species
Goat -
Specificity
This antibody is not expected to cross-react with other DGAT members. -
Tested applications
Suitable for: Sandwich ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
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Immunogen
Synthetic peptide:
C-KLEHPTQQDIDLYH
, corresponding to C terminal amino acids 346-359 of Human DGAT2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab59493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.5 - 1 µg/ml. | |
| IHC-P | Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
Target
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Function
Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. -
Tissue specificity
Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin. -
Pathway
Glycerolipid metabolism; triacylglycerol biosynthesis. -
Sequence similarities
Belongs to the diacylglycerol acyltransferase family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 404129 Cow
- Entrez Gene: 84649 Human
- Entrez Gene: 67800 Mouse
- Entrez Gene: 252900 Rat
- SwissProt: Q70VZ8 Cow
- SwissProt: Q96PD7 Human
- SwissProt: Q9DCV3 Mouse
- SwissProt: Q5FVP8 Rat
see all -
Alternative names
- DGAT2 antibody
- DGAT2_HUMAN antibody
- Diacylglycerol O acyltransferase like protein 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT2 antibody (ab59493)ab59493 at 3ug/ml staining DGAT2 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval by microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.
Datasheets and documents
References
This product has been referenced in:
- Jia H et al. Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly. J Dairy Sci 102:833-845 (2019). Read more (PubMed: 30415861) »
- Alonso C et al. Metabolomic Identification of Subtypes of Nonalcoholic Steatohepatitis. Gastroenterology 152:1449-1461.e7 (2017). Read more (PubMed: 28132890) »
