- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-DGAT2 antibody
See all DGAT2 primary antibodies -
DescriptionGoat polyclonal to DGAT2
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Host speciesGoat
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SpecificityThis antibody is not expected to cross-react with other DGAT members.
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Tested applicationsSuitable for: Sandwich ELISA, IHC-P, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
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Immunogen
Synthetic peptide:
C-KLEHPTQQDIDLYH
, corresponding to C terminal amino acids 346-359 of Human DGAT2
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab59493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.5 - 1 µg/ml. | |
| IHC-P | Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.3 - 1 µg/ml. |
Target
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FunctionEssential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation.
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Tissue specificityPredominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.
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PathwayGlycerolipid metabolism; triacylglycerol biosynthesis.
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Sequence similaritiesBelongs to the diacylglycerol acyltransferase family.
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Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
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Database links
- Entrez Gene: 404129 Cow
- Entrez Gene: 84649 Human
- Entrez Gene: 67800 Mouse
- Entrez Gene: 252900 Rat
- SwissProt: Q70VZ8 Cow
- SwissProt: Q96PD7 Human
- SwissProt: Q9DCV3 Mouse
- SwissProt: Q5FVP8 Rat
see all -
Alternative names
- DGAT2 antibody
- DGAT2_HUMAN antibody
- Diacylglycerol O acyltransferase like protein 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT2 antibody (ab59493)ab59493 at 3ug/ml staining DGAT2 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval by microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.
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Western blot - Anti-DGAT2 antibody (ab59493)All lanes : Anti-DGAT2 antibody (ab59493) at 0.3 µg/ml
Lane 1 : Cell lysates prepared from DGAT2 transiently transfected HEK293 cells.
Lane 2 : Cell lysates prepared from mock transfected HEK293 cells.
Lane 3 : Tissue lysates prepared from human liver tissue.
Observed band size: 45,60 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-DGAT2 antibody (ab59493)Anti-DGAT2 antibody (ab59493) at 0.5 µg/ml + Human Liver lysate at 35 µg
Primary incubation was 1 hour.
References
ab59493 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"