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    dgat2-antibody-ab59493.pdf

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Anti-DGAT2 antibody (ab59493)

  • Datasheet
Reviews (3) Submit a question References (7)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT2 antibody (ab59493)

    Key features and details

    • Goat polyclonal to DGAT2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Protein
    Product image
    Recombinant Human DGAT2 protein (ab153553)

    View more associated products

    Overview

    • Product name

      Anti-DGAT2 antibody
      See all DGAT2 primary antibodies
    • Description

      Goat polyclonal to DGAT2
    • Host species

      Goat
    • Specificity

      This antibody is not expected to cross-react with other DGAT members.
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      C-KLEHPTQQDIDLYH

      , corresponding to C terminal amino acids 346-359 of Human DGAT2
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • ER
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Lipids / Lipoproteins
      • Fatty Acids
      • Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Fatty acids
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Fatty acid oxidation

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human DGAT2 protein (ab153553)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab59493 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    All applications
    Mouse
    Rat
    Cow
    Dog
    Application Abreviews Notes
    IHC-P
    Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

    Target

    • Function

      Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation.
    • Tissue specificity

      Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.
    • Pathway

      Glycerolipid metabolism; triacylglycerol biosynthesis.
    • Sequence similarities

      Belongs to the diacylglycerol acyltransferase family.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession Q96PD7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 404129 Cow
      • Entrez Gene: 84649 Human
      • Entrez Gene: 67800 Mouse
      • Entrez Gene: 252900 Rat
      • SwissProt: Q70VZ8 Cow
      • SwissProt: Q96PD7 Human
      • SwissProt: Q9DCV3 Mouse
      • SwissProt: Q5FVP8 Rat
      • Unigene: 129798 Human
      • Unigene: 180189 Human
      • Unigene: 334305 Human
      • Unigene: 180189 Mouse
      • Unigene: 9523 Rat
      see all
    • Alternative names

      • DGAT2 antibody
      • DGAT2_HUMAN antibody
      • Diacylglycerol O acyltransferase like protein 2 antibody
      • Diacylglycerol O-acyltransferase 2 antibody
      • Diacylglycerol O-acyltransferase homolog 2 (mouse) antibody
      • Diacylglycerol O-acyltransferase homolog 2 antibody
      • Diacylglycerol O-acyltransferase-like protein 2 isoform 1 antibody
      • Diglyceride acyltransferase 2 antibody
      • DKFZp686A15125 antibody
      • GS1999full antibody
      • HMFN1045 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT2 antibody (ab59493)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGAT2 antibody (ab59493)

      ab59493 at 3ug/ml staining DGAT2 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval by microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (7)

    Publishing research using ab59493? Please let us know so that we can cite the reference in this datasheet.

    ab59493 has been referenced in 7 publications.

    • O'Brien PD  et al. Integrated lipidomic and transcriptomic analyses identify altered nerve triglycerides in mouse models of prediabetes and type 2 diabetes. Dis Model Mech 13:N/A (2020). PubMed: 31822493
    • Jia H  et al. Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly. J Dairy Sci 102:833-845 (2019). PubMed: 30415861
    • Howie D  et al. A Novel Role for Triglyceride Metabolism in Foxp3 Expression. Front Immunol 10:1860 (2019). PubMed: 31456800
    • Balaban S  et al. Extracellular Fatty Acids Are the Major Contributor to Lipid Synthesis in Prostate Cancer. Mol Cancer Res 17:949-962 (2019). PubMed: 30647103
    • Nagarajan SR  et al. Lipid and glucose metabolism in hepatocyte cell lines and primary mouse hepatocytes: a comprehensive resource for in vitro studies of hepatic metabolism. Am J Physiol Endocrinol Metab 316:E578-E589 (2019). PubMed: 30694691
    • Trogen G  et al. Transgenic overexpression of CTRP3 prevents alcohol-induced hepatic triglyceride accumulation. Am J Physiol Endocrinol Metab 315:E949-E960 (2018). PubMed: 29763374
    • Alonso C  et al. Metabolomic Identification of Subtypes of Nonalcoholic Steatohepatitis. Gastroenterology 152:1449-1461.e7 (2017). PubMed: 28132890

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-DGAT2 antibody

    Average
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
    Total protein in input
    500 µg
    Specification
    Hepa1-6 hepatoma cells
    Immuno-precipitation step
    Protein A
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 05 2012

    Western blot abreview for Anti-DGAT2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
    Loading amount
    40 µg
    Specification
    Hepa1-6 hepatoma cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 17 2012

    Western blot abreview for Anti-DGAT2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse muscle)
    Loading amount
    40 µg
    Specification
    Mouse muscle
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 02 2011

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