Key features and details
- Rabbit polyclonal to DGCR14
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DGCR14 antibody
DescriptionRabbit polyclonal to DGCR14
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: DGCR14 with C-terminal myc-DDK tag expressed in HEK-293T, whole cell lysate. IHC-P: Human testis, cerebral cortex, and colon tissue. ICC/IF: U-2 OS cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 53 kDa.|
FunctionMay be involved in pre-mRNA splicing.
Tissue specificityHighly expressed in heart, brain and skeletal muscle. Detected at low levels in placenta.
Sequence similaritiesBelongs to the DGCR14 family.
- Information by UniProt
- DGC14_HUMAN antibody
- DGCR13 antibody
- DGCR14 antibody
All lanes : Anti-DGCR14 antibody (ab246855) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : DGCR14 with C-terminal myc-DDK tag expressed in HEK-293T, whole cell lysate.
Predicted band size: 53 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for DGCR14 (green) using ab246855 at 4 μg/ml in ICC/IF.
Paraffin-embedded human colon tissue stained for DGCR14 using ab246855 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for DGCR14 using ab246855 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebral cortex tissue stained for DGCR14 using ab246855 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for DGCR14 using ab246855 at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246855 has not yet been referenced specifically in any publications.