Anti-DGCR6L antibody (ab229147)
Key features and details
- Rabbit polyclonal to DGCR6L
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DGCR6L antibody -
Description
Rabbit polyclonal to DGCR6L -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment within Human DGCR6L (internal sequence). The exact sequence is proprietary.
Database link: Q9BY27 -
Positive control
- WB: Mouse liver tissue extract. ICC/IF: HCT 116 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 25 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
May play a role in neural crest cell migration into the third and fourth pharyngeal pouches. -
Tissue specificity
Widely expressed in fetal and adult tissues. Highest expression in liver, heart and skeletal muscle. Lower levels in pancreas and placenta. Weak expression in brain. -
Sequence similarities
Belongs to the gonadal family. -
Cellular localization
Nucleus. Predominantly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 100126445 Cow
- Entrez Gene: 85359 Human
- Omim: 609459 Human
- SwissProt: Q9BY27 Human
- Unigene: 410965 Human
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Alternative names
- DGC6L_HUMAN antibody
- DGCR6L antibody
- DiGeorge syndrome critical region 6-like protein antibody
- Protein DGCR6L antibody
Images
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Anti-DGCR6L antibody (ab229147) at 1/1000 dilution + Mouse liver tissue extract at 50 µg
Predicted band size: 25 kDa12% SDS-PAGE gel.
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4% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells stained for DGCR6L (green) using ab229147 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229147 has not yet been referenced specifically in any publications.