Anti-DGCR8 antibody (ab90649)
Key features and details
- Rabbit polyclonal to DGCR8
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DGCR8 antibody
See all DGCR8 primary antibodies -
Description
Rabbit polyclonal to DGCR8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP Human
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Immunogen
Synthetic peptide corresponding to a region between residues 400 and 450 of Human DGCR8 (NP_073557.3).
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| IP |
Human
|
| All applications |
Horse
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
| Notes |
|---|
|
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 WW domain. -
Domain
Both DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has an autoinhibitory function on pri-miRNA processing activity. -
Cellular localization
Nucleus. Nucleus > nucleolus. Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies. - Information by UniProt
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Database links
- Entrez Gene: 54487 Human
- Omim: 609030 Human
- SwissProt: Q8WYQ5 Human
- Unigene: 643452 Human
- Unigene: 713579 Human
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Alternative names
- DGCRK6 antibody
- C22orf12 antibody
- D16H22S788E antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGCR8 antibody (ab90649)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DGCR8 with ab90649 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-DGCR8 antibody (ab90649)Detection of Human DGCR8 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab90649 at 3 µg/mg lysate for IP (Lane 1). Lane 2 represents control IgG IP.
ab90579, which recognizes an upstream epitope, was used at 1 µg/ml for subsequent WB detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
References (0)
ab90649 has not yet been referenced specifically in any publications.
