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    dgcr8-antibody-ab90649.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing RNAi
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Anti-DGCR8 antibody (ab90649)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGCR8 antibody (ab90649)
  • Immunoprecipitation - Anti-DGCR8 antibody (ab90649)

Key features and details

  • Rabbit polyclonal to DGCR8
  • Suitable for: IHC-P, IP
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human DGCR8 protein (ab153373)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-DGCR8 antibody
    See all DGCR8 primary antibodies
  • Description

    Rabbit polyclonal to DGCR8
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to a region between residues 400 and 450 of Human DGCR8 (NP_073557.3).

  • Positive control

    • HeLa whole cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Dicer
    • Epigenetics and Nuclear Signaling
    • RNAi
    • miRNA processing
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human DGCR8 protein (ab153373)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab90649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
IP
Human
All applications
Horse
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Application Abreviews Notes
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at 2-5 µg/mg of lysate.
Notes
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at 2-5 µg/mg of lysate.

Target

  • Function

    Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 WW domain.
  • Domain

    Both DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has an autoinhibitory function on pri-miRNA processing activity.
  • Cellular localization

    Nucleus. Nucleus > nucleolus. Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies.
  • Target information above from: UniProt accession Q8WYQ5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 54487 Human
    • Omim: 609030 Human
    • SwissProt: Q8WYQ5 Human
    • Unigene: 643452 Human
    • Unigene: 713579 Human
    • Alternative names

      • DGCRK6 antibody
      • C22orf12 antibody
      • D16H22S788E antibody
      • D16Wis2 antibody
      • DGCR 8 antibody
      • Dgcr8 antibody
      • DGCR8 microprocessor complex subunit antibody
      • DGCR8_HUMAN antibody
      • DGCRK 6 antibody
      • DiGeorge syndrome critical region 8 antibody
      • DiGeorge syndrome critical region gene 8 antibody
      • Gy1 antibody
      • Microprocessor complex subunit DGCR8 antibody
      • pasha antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGCR8 antibody (ab90649)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DGCR8 antibody (ab90649)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DGCR8 with ab90649 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunoprecipitation - Anti-DGCR8 antibody (ab90649)
      Immunoprecipitation - Anti-DGCR8 antibody (ab90649)
      Detection of Human DGCR8 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab90649 at 3 µg/mg lysate for IP (Lane 1). Lane 2 represents control IgG IP.
      ab90579, which recognizes an upstream epitope, was used at 1 µg/ml for subsequent WB detection.
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab90649? Please let us know so that we can cite the reference in this datasheet.

    ab90649 has not yet been referenced specifically in any publications.

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