Overview

  • Product name

    Anti-DGCR8 antibody - N-terminal
    See all DGCR8 primary antibodies
  • Description

    Rabbit polyclonal to DGCR8 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human DGCR8 (N terminal). The exact sequence is proprietary.
    Database link: Q8WYQ5

  • Positive control

    • WB: HEK-293T and A431 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab227585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 86 kDa.

Target

  • Function

    Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 WW domain.
  • Domain

    Both DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has an autoinhibitory function on pri-miRNA processing activity.
  • Cellular localization

    Nucleus. Nucleus > nucleolus. Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • DGCRK6 antibody
    • C22orf12 antibody
    • D16H22S788E antibody
    • D16Wis2 antibody
    • DGCR 8 antibody
    • Dgcr8 antibody
    • DGCR8 microprocessor complex subunit antibody
    • DGCR8_HUMAN antibody
    • DGCRK 6 antibody
    • DiGeorge syndrome critical region 8 antibody
    • DiGeorge syndrome critical region gene 8 antibody
    • Gy1 antibody
    • Microprocessor complex subunit DGCR8 antibody
    • pasha antibody
    see all

Images

  • All lanes : Anti-DGCR8 antibody - N-terminal (ab227585) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 86 kDa



    7.5% SDS-PAGE

References

ab227585 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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