Key features and details
- Rabbit polyclonal to DGKA
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DGKA antibody
See all DGKA primary antibodies
DescriptionRabbit polyclonal to DGKA
SpecificityThis antibody detects endogenous levels of total DGKA protein.
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to internal sequence amino acids 331-370 of Human DGKA
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).|
FunctionUpon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.
Tissue specificityLymphocytes and oligodendroglial cells.
Sequence similaritiesBelongs to the eukaryotic diacylglycerol kinase family.
Contains 1 DAGKc domain.
Contains 2 EF-hand domains.
Contains 2 phorbol-ester/DAG-type zinc fingers.
- Information by UniProt
- 80 kDa diacylglycerol kinase antibody
- DAG kinase alpha antibody
- DAGK antibody
All lanes : Anti-DGKA antibody (ab64845) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with blocking peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
ICC/IF image of ab64845 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64845, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab64845 has been referenced in 2 publications.
- Jung IY et al. CRISPR/Cas9-Mediated Knockout of DGK Improves Antitumor Activities of Human T Cells. Cancer Res 78:4692-4703 (2018). PubMed: 29967261
- Ruffo E et al. Inhibition of diacylglycerol kinase a restores restimulation-induced cell death and reduces immunopathology in XLP-1. Sci Transl Med 8:321ra7 (2016). Human . PubMed: 26764158