Product nameAnti-DGKA antibody
See all DGKA primary antibodies
DescriptionRabbit polyclonal to DGKA
SpecificityThis antibody detects endogenous levels of total DGKA protein.
Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to internal sequence amino acids 331-370 of Human DGKA
- Extracts from Jurkat cells. IHC-P (FFPE): Human Skin (Normal) tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).|
|IHC-P||Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionUpon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.
Tissue specificityLymphocytes and oligodendroglial cells.
Sequence similaritiesBelongs to the eukaryotic diacylglycerol kinase family.
Contains 1 DAGKc domain.
Contains 2 EF-hand domains.
Contains 2 phorbol-ester/DAG-type zinc fingers.
- Information by UniProt
- 80 kDa diacylglycerol kinase antibody
- DAG kinase alpha antibody
- DAGK antibody
IHC image of DGKA staining in Human Skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64845, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
All lanes : Anti-DGKA antibody (ab64845) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with blocking peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
ICC/IF image of ab64845 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64845, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.