Overview

  • Product name

  • Description

    Mouse polyclonal to DGKA
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length Human protein (NP_001336.2)

  • Positive control

    • Human spleen lysate; Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.
  • Tissue specificity

    Lymphocytes and oligodendroglial cells.
  • Sequence similarities

    Belongs to the eukaryotic diacylglycerol kinase family.
    Contains 1 DAGKc domain.
    Contains 2 EF-hand domains.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
  • Information by UniProt
  • Database links

  • Alternative names

    • 80 kDa diacylglycerol kinase antibody
    • DAG kinase alpha antibody
    • DAGK antibody
    • DAGK1 antibody
    • DGK alpha antibody
    • DGK-alpha antibody
    • dgkA antibody
    • DGKA_HUMAN antibody
    • Diacylglycerol kinase alpha antibody
    • Diacylglycerol kinase, alpha 80kDa antibody
    • Diglyceride kinase alpha antibody
    • MGC12821 antibody
    • MGC42356 antibody
    • OTTHUMP00000242836 antibody
    • OTTHUMP00000242955 antibody
    • OTTHUMP00000244046 antibody
    see all

Images

  • Anti-DGKA antibody (ab88672) at 1 µg/ml + Human spleen lysate at 50 µg

    Predicted band size: 83 kDa

  • All lanes : Anti-DGKA antibody (ab88672) at 1 µg/ml

    Lane 1 : DGKA transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 83 kDa
    Observed band size: 81 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence staining of DGKA in HeLa cells using ab88672 at 10 µg/ml.

References

This product has been referenced in:

  • Lupino E  et al. I?B kinase ß is required for activation of NF-?B and AP-1 in CD3/CD28-stimulated primary CD4(+) T cells. J Immunol 188:2545-55 (2012). Human . Read more (PubMed: 22331067) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

To our knowledge, ab88672 has not been tested in baboon. However, the human DGKA protein sequence has 99% homology with the baboon DGKA sequence so the antibody should be able to recognize baboon DGKA. By participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More
Abreviews
Application
Western blot
Sample
Mouse Recombinant protein (lysate of Cos'7 overexpressing GFP-human, mouse,)
Loading amount
75 µg
Specification
lysate of Cos'7 overexpressing GFP-human, mouse,
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. Y Shirai

Verified customer

Submitted Aug 05 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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