Recombinant Anti-DGKE antibody [EPR7847] (ab128949)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7847] to DGKE
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DGKE antibody [EPR7847]
See all DGKE primary antibodies -
Description
Rabbit monoclonal [EPR7847] to DGKE -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, HeLa and K562 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.09 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7847 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
Target
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Function
Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition. -
Tissue specificity
Expressed predominantly in testis. -
Sequence similarities
Belongs to the eukaryotic diacylglycerol kinase family.
Contains 1 DAGKc domain.
Contains 2 phorbol-ester/DAG-type zinc fingers. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8526 Human
- Omim: 601440 Human
- SwissProt: P52429 Human
- Unigene: 239514 Human
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Alternative names
- AHUS7 antibody
- DAG kinase epsilon antibody
- DAGK 5 antibody
see all
Images
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All lanes : Anti-DGKE antibody [EPR7847] (ab128949) at 1/1000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates prepared in RIPA lysis method
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
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All lanes : Anti-DGKE antibody [EPR7847] (ab128949) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaThe cell lysates were prepared in 1%SDS Hot lysis method.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab128949 has not yet been referenced specifically in any publications.