Overview

  • Product name

    Anti-DGKE antibody [EPR7847]
    See all DGKE primary antibodies
  • Description

    Rabbit monoclonal [EPR7847] to DGKE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human DGKE (UniProt P52429).

  • Positive control

    • 293T, HeLa and K562 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
    • Tissue specificity

      Expressed predominantly in testis.
    • Sequence similarities

      Belongs to the eukaryotic diacylglycerol kinase family.
      Contains 1 DAGKc domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AHUS7 antibody
      • DAG kinase epsilon antibody
      • DAGK 5 antibody
      • DAGK 6 antibody
      • DAGK5 antibody
      • DAGK6 antibody
      • DGK antibody
      • DGK epsilon antibody
      • DGK-epsilon antibody
      • DGKE antibody
      • DGKE_HUMAN antibody
      • Diacylglycerol kinase epsilon 64kDa antibody
      • Diacylglycerol kinase epsilon antibody
      • Diglyceride kinase epsilon antibody
      • NPHS7 antibody
      see all

    Images

    • All lanes : Anti-DGKE antibody [EPR7847] (ab128949) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Hela cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab128949 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab128949.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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