Product nameAnti-DGKE antibody [EPR7847]
See all DGKE primary antibodies
DescriptionRabbit monoclonal [EPR7847] to DGKE
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
A synthetic peptide corresponding to residues in Human DGKE (UniProt P52429).
- 293T, HeLa and K562 cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.09 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab128949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).|
|ICC/IF||1/50 - 1/100.|
FunctionHighly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Tissue specificityExpressed predominantly in testis.
Sequence similaritiesBelongs to the eukaryotic diacylglycerol kinase family.
Contains 1 DAGKc domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
- Information by UniProt
- AHUS7 antibody
- DAG kinase epsilon antibody
- DAGK 5 antibody
All lanes : Anti-DGKE antibody [EPR7847] (ab128949) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Hela cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab128949 has not yet been referenced specifically in any publications.