Overview

  • Product name

  • Description

    Rabbit polyclonal to DGKG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 469 and 694 of Human DGKG (Uniprot ID: P49619)

  • Positive control

    • A431, H1299, HeLa, HepG2 or Raji whole cell lysate, HeLa cells, NCIN87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab151967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 89 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    DGKG (Diacylglycerol kinase gamma ) reverses the normal flow of glycerolipid biosynthesis by phosphorylating diacylglycerol back to phosphatidic acid. This enzyme initiates resynthesis of phosphoinositides consumed by phospholipase C during cellular signal transduction. DGK gamma is one of multiple DGK isozymes found in animal cells. It is expressed abundantly in the human retina and to a much lesser extent in the brain. Other tissues contain extremely low levels.
  • Cellular localization

    Cell Membrane and Cytoplasmic.
  • Database links

  • Alternative names

    • DGKG antibody
    • DAG kinase gamma antibody
    • DAGK3 antibody
    • DGK gamma antibody
    • diacylglycerol kinase gamma 90kDa antibody
    • Diacylglycerol kinase gamma antibody
    • Diglyceride kinase antibody
    • Diglyceride kinase gamma antibody
    see all

Images

  • Anti-DGKG antibody (ab151967) at 1/1000 dilution + H1299 lysate at 30 µg

    Predicted band size: 89 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling DGKG with ab151967 a 1/200 dilution.
  • Immunohistochemical analysis of paraffin embedded Human NCIN87 xenograft tissue labeling DGKG with ab151967 at a 1/500 dilution.

References

ab151967 has not yet been referenced specifically in any publications.

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