Key features and details
- Rabbit polyclonal to DGKG
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DGKG antibody
See all DGKG primary antibodies
DescriptionRabbit polyclonal to DGKG
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to a region within amino acids 469 and 694 of Human DGKG (Uniprot ID: P49619)
- A431, H1299, HeLa, HepG2 or Raji whole cell lysate, HeLa cells, NCIN87 xenograft tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 89 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
RelevanceDGKG (Diacylglycerol kinase gamma ) reverses the normal flow of glycerolipid biosynthesis by phosphorylating diacylglycerol back to phosphatidic acid. This enzyme initiates resynthesis of phosphoinositides consumed by phospholipase C during cellular signal transduction. DGK gamma is one of multiple DGK isozymes found in animal cells. It is expressed abundantly in the human retina and to a much lesser extent in the brain. Other tissues contain extremely low levels.
Cellular localizationCell Membrane and Cytoplasmic.
- DGKG antibody
- DAG kinase gamma antibody
- DAGK3 antibody
Anti-DGKG antibody (ab151967) at 1/1000 dilution + H1299 lysate at 30 µg
Predicted band size: 89 kDa
7.5% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling DGKG with ab151967 a 1/200 dilution.
Immunohistochemical analysis of paraffin embedded Human NCIN87 xenograft tissue labeling DGKG with ab151967 at a 1/500 dilution.
ab151967 has not yet been referenced specifically in any publications.