Overview

  • Product name

    Anti-DGKZ/DGK-zeta antibody
    See all DGKZ/DGK-zeta primary antibodies
  • Description

    Rabbit polyclonal to DGKZ/DGK-zeta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis, Medaka fish
  • Immunogen

    Synthetic peptide corresponding to Human DGKZ/DGK-zeta aa 1067-1117.
    Database link: NP_001099010.1

  • Positive control

    • HeLa and 293T whole cell lysate
  • General notes

    Previously labelled as DGKZ.

Properties

Applications

Our Abpromise guarantee covers the use of ab105195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity.
    • Tissue specificity

      Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle.
    • Sequence similarities

      Belongs to the eukaryotic diacylglycerol kinase family.
      Contains 2 ANK repeats.
      Contains 1 DAGKc domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
    • Post-translational
      modifications

      Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.
    • Cellular localization

      Cytoplasm. Nucleus. Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • DAG kinase zeta antibody
      • DAGK5 antibody
      • DAGK5 PEN antibody
      • DAGK6 antibody
      • DGK ZETA antibody
      • DGK-zeta antibody
      • Dgkz antibody
      • DGKZ_HUMAN antibody
      • Diacylglycerol kinase zeta antibody
      • Diglyceride kinase zeta antibody
      • hDGKzeta antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DGKZ/DGK-zeta with ab105195 at 1/1000 (1µg/ml). Detection: DAB.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DGKZ/DGK-zeta with ab105195 at 1/1000 (1µg/ml). Detection: DAB.

    • All lanes : Anti-DGKZ/DGK-zeta antibody (ab105195) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 124 kDa


      Exposure time: 30 seconds

    References

    This product has been referenced in:

    • Torres-Ayuso P  et al. Diacylglycerol kinase-? regulates mTORC1 and lipogenic metabolism in cancer cells through SREBP-1. Oncogenesis 4:e164 (2015). Read more (PubMed: 26302180) »
    • Gharbi SI  et al. Transient PKCa shuttling to the immunological synapse is governed by DGK? and regulates L-selectin shedding. J Cell Sci 126:2176-86 (2013). Read more (PubMed: 23525016) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us.

    The predicted band size according to Swissprot is 124kDa. http://www.uniprot.org/uniprot/Q13574

    The observed band size will be slightly different due to post translational modifications and processing of proteins. We recommend using Hela and 293T cell lysates as positive control when using this antibody in western blot.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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