Key features and details
- Rabbit polyclonal to DGKZ/DGK-zeta
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DGKZ/DGK-zeta antibody
See all DGKZ/DGK-zeta primary antibodies
DescriptionRabbit polyclonal to DGKZ/DGK-zeta
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis, Medaka fish
Synthetic peptide corresponding to Human DGKZ/DGK-zeta aa 1067-1117.
Database link: NP_001099010.1
- HeLa and 293T whole cell lysate
Previously labelled as DGKZ.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab105195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 124 kDa.|
FunctionDisplays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity.
Tissue specificityHighest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle.
Sequence similaritiesBelongs to the eukaryotic diacylglycerol kinase family.
Contains 2 ANK repeats.
Contains 1 DAGKc domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
modificationsPhosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.
Cellular localizationCytoplasm. Nucleus. Cell membrane.
- Information by UniProt
- DAG kinase zeta antibody
- DAGK5 antibody
- DAGK5 PEN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DGKZ/DGK-zeta with ab105195 at 1/1000 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DGKZ/DGK-zeta with ab105195 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-DGKZ/DGK-zeta antibody (ab105195) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30 seconds
ab105195 has been referenced in 4 publications.
- Stangl A et al. Regulation of the endosomal SNX27-retromer by OTULIN. Nat Commun 10:4320 (2019). PubMed: 31541095
- Jung IY et al. CRISPR/Cas9-Mediated Knockout of DGK Improves Antitumor Activities of Human T Cells. Cancer Res 78:4692-4703 (2018). PubMed: 29967261
- Torres-Ayuso P et al. Diacylglycerol kinase-? regulates mTORC1 and lipogenic metabolism in cancer cells through SREBP-1. Oncogenesis 4:e164 (2015). PubMed: 26302180
- Gharbi SI et al. Transient PKCa shuttling to the immunological synapse is governed by DGK? and regulates L-selectin shedding. J Cell Sci 126:2176-86 (2013). PubMed: 23525016