Product nameAnti-DGKZ/DGK-zeta antibody
See all DGKZ/DGK-zeta primary antibodies
DescriptionRabbit polyclonal to DGKZ/DGK-zeta
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis, Medaka fish
Synthetic peptide corresponding to Human DGKZ/DGK-zeta aa 1067-1117.
Database link: NP_001099010.1
- HeLa and 293T whole cell lysate
Previously labelled as DGKZ.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab105195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 124 kDa.|
FunctionDisplays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity.
Tissue specificityHighest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle.
Sequence similaritiesBelongs to the eukaryotic diacylglycerol kinase family.
Contains 2 ANK repeats.
Contains 1 DAGKc domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
modificationsPhosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.
Cellular localizationCytoplasm. Nucleus. Cell membrane.
- Information by UniProt
- DAG kinase zeta antibody
- DAGK5 antibody
- DAGK5 PEN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DGKZ/DGK-zeta with ab105195 at 1/1000 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DGKZ/DGK-zeta with ab105195 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-DGKZ/DGK-zeta antibody (ab105195) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30 seconds
This product has been referenced in:
- Torres-Ayuso P et al. Diacylglycerol kinase-? regulates mTORC1 and lipogenic metabolism in cancer cells through SREBP-1. Oncogenesis 4:e164 (2015). Read more (PubMed: 26302180) »
- Gharbi SI et al. Transient PKCa shuttling to the immunological synapse is governed by DGK? and regulates L-selectin shedding. J Cell Sci 126:2176-86 (2013). Read more (PubMed: 23525016) »