Recombinant
RabMAb

Recombinant Anti-DGKZ/DGK-zeta antibody [EPR22040-72] - BSA and Azide free (ab239792)

Overview

  • Product name

    Anti-DGKZ/DGK-zeta antibody [EPR22040-72] - BSA and Azide free
    See all DGKZ/DGK-zeta primary antibodies
  • Description

    Rabbit monoclonal [EPR22040-72] to DGKZ/DGK-zeta - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human DGKZ/DGK-zeta aa 700-1000. The exact sequence is proprietary.
    Database link: Q13574

  • Positive control

    • IHC-P: Human tonsil tissue.
  • General notes

    ab239792 is the carrier-free version of ab239080 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239792 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as DGKZ.

Properties

Applications

Our Abpromise guarantee covers the use of ab239792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 124 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity.
  • Tissue specificity

    Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle.
  • Sequence similarities

    Belongs to the eukaryotic diacylglycerol kinase family.
    Contains 2 ANK repeats.
    Contains 1 DAGKc domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DAG kinase zeta antibody
    • DAGK5 antibody
    • DAGK5 PEN antibody
    • DAGK6 antibody
    • DGK ZETA antibody
    • DGK-zeta antibody
    • Dgkz antibody
    • DGKZ_HUMAN antibody
    • Diacylglycerol kinase zeta antibody
    • Diglyceride kinase zeta antibody
    • hDGKzeta antibody
    see all

Images

  • DGKZ/DGK-zeta was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab239080 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab239080 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: Jurkat whole cell lysat 10 μg (input).
    Lane 2: ab239080 IP in Jurkat whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239080 in Jurkat whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 15 seconds.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239080).

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling DGKZ/DGK-zeta with ab239080 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on neurons of rat brain (PMID: 14511325) (PMID: 24119575) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239080).

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling DGKZ/DGK-zeta with ab239080 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on neurons of mouse brain (PMID: 14511325) (PMID: 24119575) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239080).

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling DGKZ/DGK-zeta with ab239080 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on Purkinje cells of human cerebellum (PMID: 14511325) (PMID: 24119575) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239080).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilzed Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling DGKZ/DGK-zeta with ab239080 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control, and an unlabeled control (Cell without incubation with primary antibody and secondary antibody, Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239080).

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling DGKZ/DGK-zeta with ab239080 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic Cytoplasmic staining on subset of immune cells in human tonsil (PMID: 24573202) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239080).

     

References

ab239792 has not yet been referenced specifically in any publications.

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