Overview

  • Product name

  • Description

    Rabbit polyclonal to DHCR7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human DHCR7 aa 1-50. The exact sequence is proprietary.
    Database link: Q9UBM7

  • Positive control

    • IP: HEK-293T whole cell lysate.
  • General notes

    ab226409 has not performed satisfactorily whenused for WB of DHCR7 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of DHCR7.

Properties

Applications

Our Abpromise guarantee covers the use of ab226409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance

      The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[OMIM]
    • Cellular localization

      Endoplasmic reticulum membrane; Multi-pass membrane protein
    • Database links

    • Alternative names

      • 7 dehydrocholesterol reductase antibody
      • 7 DHC reductase antibody
      • Delta 7 dehydrocholesterol reductase antibody
      • Putative sterol reductase SR 2 antibody
      • SLOS antibody
      • Sterol delta 7 reductase antibody
      • Sterol Delta antibody
      see all

    Images

    • DHCR7 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226409 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226409 at 1 µg/ml.

      Lane 1: ab226409 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

    References

    ab226409 has not yet been referenced specifically in any publications.

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