Product nameAnti-DHCR7 antibody
See all DHCR7 primary antibodies
DescriptionRabbit polyclonal to DHCR7
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide within Human DHCR7 aa 1-50. The exact sequence is proprietary.
Database link: Q9UBM7
- IP: HEK-293T whole cell lysate.
ab226409 has not performed satisfactorily whenused for WB of DHCR7 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of DHCR7.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226409 was affinity purified using an epitope specific to DHCR7 immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab226409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceThe DHCR7 gene encodes delta-7-sterol reductase (EC 220.127.116.11), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[OMIM]
Cellular localizationEndoplasmic reticulum membrane; Multi-pass membrane protein
- 7 dehydrocholesterol reductase antibody
- 7 DHC reductase antibody
- Delta 7 dehydrocholesterol reductase antibody
DHCR7 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226409 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226409 at 1 µg/ml.
Lane 1: ab226409 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab226409 has not yet been referenced specifically in any publications.