Product nameAnti-DHCR7 antibody - C-terminal
See all DHCR7 primary antibodies
DescriptionRabbit polyclonal to DHCR7 - C-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant fragment within Human DHCR7 (C terminal). The exact sequence is proprietary.
Database link: Q9UBM7
- IHC-P: Mouse liver and rat duodenum tissues. WB: HepG2 whole cell and membrane extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab226784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 54 kDa.|
RelevanceThe DHCR7 gene encodes delta-7-sterol reductase (EC 18.104.22.168), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[OMIM]
Cellular localizationEndoplasmic reticulum membrane; Multi-pass membrane protein
- 7 dehydrocholesterol reductase antibody
- 7 DHC reductase antibody
- Delta 7 dehydrocholesterol reductase antibody
All lanes : Anti-DHCR7 antibody - C-terminal (ab226784) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract, boiled
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) membrane extract, boiled
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract, unboiled
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) membrane extract, unboiled
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded mouse liver tissue stained for DHCR7 using ab226784 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat duodenum tissue stained for DHCR7 using ab226784 at 1/500 dilution in immunohistochemical analysis.
ab226784 has not yet been referenced specifically in any publications.