Key features and details
- Rabbit polyclonal to DHCR7 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DHCR7 antibody - C-terminal
See all DHCR7 primary antibodies
DescriptionRabbit polyclonal to DHCR7 - C-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant fragment within Human DHCR7 (C terminal). The exact sequence is proprietary.
Database link: Q9UBM7
- IHC-P: Mouse liver and rat duodenum tissues. WB: HepG2 whole cell and membrane extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab226784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 54 kDa.|
RelevanceThe DHCR7 gene encodes delta-7-sterol reductase (EC 188.8.131.52), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[OMIM]
Cellular localizationEndoplasmic reticulum membrane; Multi-pass membrane protein
- 7 dehydrocholesterol reductase antibody
- 7 DHC reductase antibody
- Delta 7 dehydrocholesterol reductase antibody
All lanes : Anti-DHCR7 antibody - C-terminal (ab226784) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract, boiled
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) membrane extract, boiled
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract, unboiled
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) membrane extract, unboiled
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded mouse liver tissue stained for DHCR7 using ab226784 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat duodenum tissue stained for DHCR7 using ab226784 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226784 has not yet been referenced specifically in any publications.