Overview

  • Product name

    Anti-DHCR7 antibody - C-terminal
    See all DHCR7 primary antibodies
  • Description

    Rabbit polyclonal to DHCR7 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human DHCR7 (C terminal). The exact sequence is proprietary.
    Database link: Q9UBM7

  • Positive control

    • IHC-P: Mouse liver and rat duodenum tissues. WB: HepG2 whole cell and membrane extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab226784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.

Target

  • Relevance

    The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[OMIM]
  • Cellular localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein
  • Database links

  • Alternative names

    • 7 dehydrocholesterol reductase antibody
    • 7 DHC reductase antibody
    • Delta 7 dehydrocholesterol reductase antibody
    • Putative sterol reductase SR 2 antibody
    • SLOS antibody
    • Sterol delta 7 reductase antibody
    • Sterol Delta antibody
    see all

Images

  • All lanes : Anti-DHCR7 antibody - C-terminal (ab226784) at 1/1000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract, boiled
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) membrane extract, boiled
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract, unboiled
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) membrane extract, unboiled

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 54 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded mouse liver tissue stained for DHCR7 using ab226784 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat duodenum tissue stained for DHCR7 using ab226784 at 1/500 dilution in immunohistochemical analysis.

References

ab226784 has not yet been referenced specifically in any publications.

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