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As we have to prepare the sample 1: 50 dilution (20 ul of serum + 980ul of 1X Serum diluent) before add onto the plate wells, so for the final results, should we multiple it ?
For example : sample result was 1.365 ug/ml, and the normal range of adults is 0.9-3.6 ug/ml (by abcam)
If we multiple it, 1.365 x 50 = 68.25 ug/ml, it did not make sense.
Asked on Mar 24 2015