Overview

  • Product name

    Anti-Dhh antibody [RM0086-6A12]
    See all Dhh primary antibodies
  • Description

    Rat monoclonal [RM0086-6A12] to Dhh
  • Host species

    Rat
  • Specificity

    ab86061 detects Mouse Dhh N terminal polypeptide, but not C terminal polypeptide.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Mouse recombinant Dhh N-terminal fragment

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and filter sterilized through a 0.2 µm filter.
  • Clonality

    Monoclonal
  • Clone number

    RM0086-6A12
  • Isotype

    IgG2
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Predicted molecular weight: Unprocessed precursor, 44 kDa; Dhh protein N-product, 20 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.
    • Involvement in disease

      Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD) [MIM:607080]. PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity.
      Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM) [MIM:233420]; also known as male-limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks.
    • Sequence similarities

      Belongs to the hedgehog family.
    • Post-translational
      modifications

      The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    • Cellular localization

      Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
    • Information by UniProt
    • Database links

    • Alternative names

      • C78960 antibody
      • Desert hedgehog (Drosophila) homolog antibody
      • Desert hedgehog antibody
      • Desert hedgehog homolog (Drosophila) antibody
      • Desert hedgehog protein C-product antibody
      • Desert hedgehog protein precursor antibody
      • DHH antibody
      • DHH_HUMAN antibody
      • GDXYM antibody
      • HHG-3 antibody
      • Hira antibody
      • SRXY7 antibody
      see all

    References

    This product has been referenced in:

    • Daane JM & Downs KM Hedgehog signaling in the posterior region of the mouse gastrula suggests manifold roles in the fetal-umbilical connection and posterior morphogenesis. Dev Dyn 240:2175-93 (2011). Read more (PubMed: 22016185) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab86061.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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