Recombinant Anti-DHHC-18 antibody [EPR9871] (ab154790)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9871] to DHHC-18
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DHHC-18 antibody [EPR9871]
See all DHHC-18 primary antibodies -
Description
Rabbit monoclonal [EPR9871] to DHHC-18 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human DHHC-18.
Database link: Q9NUE0 -
Positive control
- HeLa, Fetal heart and Human bladder lysates; Human stomach tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9871 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Has palmitoyltransferase activity towards HRAS and LCK. -
Sequence similarities
Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.
Contains 1 DHHC-type zinc finger. -
Domain
The DHHC domain is required for palmitoyltransferase activity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 84243 Human
- Entrez Gene: 503610 Mouse
- Entrez Gene: 362613 Rat
- SwissProt: Q9NUE0 Human
- SwissProt: Q5Y5T2 Mouse
- SwissProt: Q2TGJ1 Rat
- Unigene: 523710 Human
- Unigene: 331948 Mouse
see all -
Alternative names
- DHHC-18 antibody
- DHHC18 antibody
- DKFZp667O2416 antibody
see all
Images
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All lanes : Anti-DHHC-18 antibody [EPR9871] (ab154790) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal heart lysate
Lane 3 : Human bladder lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa -
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling DHHC-18 with ab154790 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154790 has been referenced in 1 publication.
- Chen X et al. DHHC protein family targets different subsets of glioma stem cells in specific niches. J Exp Clin Cancer Res 38:25 (2019). PubMed: 30658672