Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9871] to DHHC-18
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-DHHC-18 antibody [EPR9871]
See all DHHC-18 primary antibodies
DescriptionRabbit monoclonal [EPR9871] to DHHC-18
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human DHHC-18.
Database link: Q9NUE0
- HeLa, Fetal heart and Human bladder lysates; Human stomach tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionHas palmitoyltransferase activity towards HRAS and LCK.
Sequence similaritiesBelongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.
Contains 1 DHHC-type zinc finger.
DomainThe DHHC domain is required for palmitoyltransferase activity.
- Information by UniProt
- DHHC-18 antibody
- DHHC18 antibody
- DKFZp667O2416 antibody
All lanes : Anti-DHHC-18 antibody [EPR9871] (ab154790) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal heart lysate
Lane 3 : Human bladder lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling DHHC-18 with ab154790 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab154790 has been referenced in 1 publication.
- Chen X et al. DHHC protein family targets different subsets of glioma stem cells in specific niches. J Exp Clin Cancer Res 38:25 (2019). PubMed: 30658672