Anti-DHHC-20 antibody (ab110478)

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Western blot - Anti-DHHC-20 antibody (ab110478)

    Key features and details

    • Rabbit polyclonal to DHHC-20
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-DHHC-20 antibody

    • Description

      Rabbit polyclonal to DHHC-20

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat

    • Immunogen

      Synthetic peptide corresponding to Human DHHC-20 (internal sequence).

    • Positive control

      • Extracts from COLO, MCF7 and Jurkat cells

    • General notes

      Previously labelled as ZDHHC20. 

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)

      PBS is without Mg2+ and Ca2+.
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      ab110478 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab110478 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/1000. Predicted molecular weight: 42 kDa.

    Target

    Images

    • Western blot - Anti-DHHC-20 antibody (ab110478)
      Western blot - Anti-DHHC-20 antibody (ab110478)
      All lanes : Anti-DHHC-20 antibody (ab110478) at 1/500 dilution

      Lane 1 : Extracts from COLO cells
      Lane 2 : Extracts from MCF7 cells
      Lane 3 : Extracts from Jurkat cells
      Lane 4 : Extracts from Jurkat cells with immunized peptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 42 kDa

    References (1)

    Publishing research using ab110478? Please let us know so that we can cite the reference in this datasheet.

    ab110478 has been referenced in 1 publication.

    • Wang W  et al. Protein Depalmitoylation Is Induced by Wnt5a and Promotes Polarized Cell Behavior. J Biol Chem 290:15707-16 (2015). PubMed: 25944911

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