Anti-DHHC-20 antibody (ab110478)
Key features and details
- Rabbit polyclonal to DHHC-20
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DHHC-20 antibody -
Description
Rabbit polyclonal to DHHC-20 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human DHHC-20 (internal sequence).
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Positive control
- Extracts from COLO, MCF7 and Jurkat cells
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General notes
Previously labelled as ZDHHC20.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab110478 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab110478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/1000. Predicted molecular weight: 42 kDa. |
Target
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Sequence similarities
Belongs to the DHHC palmitoyltransferase family.
Contains 1 DHHC-type zinc finger. -
Domain
The DHHC domain is required for palmitoyltransferase activity. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 253832 Human
- Entrez Gene: 75965 Mouse
- Entrez Gene: 305923 Rat
- SwissProt: Q5W0Z9 Human
- SwissProt: Q5Y5T1 Mouse
- Unigene: 564611 Human
- Unigene: 29044 Mouse
- Unigene: 22849 Rat
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Alternative names
- 4933421L13Rik antibody
- DHHC-20 antibody
- Probable palmitoyltransferase ZDHHC20 antibody
see all
Images
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All lanes : Anti-DHHC-20 antibody (ab110478) at 1/500 dilution
Lane 1 : Extracts from COLO cells
Lane 2 : Extracts from MCF7 cells
Lane 3 : Extracts from Jurkat cells
Lane 4 : Extracts from Jurkat cells with immunized peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
Datasheets and documents
References (1)
ab110478 has been referenced in 1 publication.
- Wang W et al. Protein Depalmitoylation Is Induced by Wnt5a and Promotes Polarized Cell Behavior. J Biol Chem 290:15707-16 (2015). PubMed: 25944911