Overview

  • Product name

  • Description

    Rabbit polyclonal to DHHC-5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human DHHC-5 aa 500-550. The exact sequence is proprietary.
    Database link: Q9C0B5

  • Positive control

    • HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
  • General notes

    Previously labelled as ZDHHC5.

Properties

Applications

Our Abpromise guarantee covers the use of ab200572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 78 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-DHHC-5 antibody (ab200572) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysates
    Lane 2 : 293T whole cell lysates
    Lane 3 : Jurkat whole cell lysates

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 78 kDa


    Exposure time: 30 seconds
  • All lanes : Anti-DHHC-5 antibody (ab200572) at 0.1 µg/ml

    Lane 1 : TCMK-1 whole cell lysate
    Lane 2 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 78 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling DHHC-5 using ab200572 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated DHHC-5, ab200572 was used at 0.4 μg/ml.

References

ab200572 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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