Overview

  • Product name
    Anti-DHI1L antibody - C-terminal
  • Description
    Rabbit polyclonal to DHI1L - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DHI1L aa 263-291 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q7Z5J1

  • Positive control
    • K562 cell lysate; Human prostate carcinoma tissue; U-251 MG cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 34 kDa.
ICC/IF 1/10 - 1/50.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 11-beta-HSD3 antibody
    • 11-beta-hydroxysteroid dehydrogenase type 3 antibody
    • 11-DH3 antibody
    • DHI1L_HUMAN antibody
    • HSD11B1L antibody
    • HSD3 antibody
    • Hydroxysteroid 11-beta-dehydrogenase 1-like protein antibody
    • SCDR10 antibody
    • SDR26C2 antibody
    • short chain dehydrogenase/reductase family 26C, member 2 antibody
    • Short-chain dehydrogenase/reductase 10 antibody
    see all

Images

  • Anti-DHI1L antibody - C-terminal (ab170372) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size: 34 kDa

  • Immunofluorescent analysis of U-251 MG cells labeling DHI1L with ab170372 at a 1/10 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling DHI1L with ab170372 at a 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab170372 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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