Product nameAnti-DHODH antibody
See all DHODH primary antibodies
DescriptionRabbit polyclonal to DHODH
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human DHODH aa 280-395 (C terminal).
Database link: Q02127
- MCF7, HL60, SW620 and HepG2 cell extracts; Mouse heart, spleen and kidney extracts; Rat heart and kidney extracts; Human esophagus, colon, gastric and liver cancer tissues; Mouse brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
- HepG2 whole cell lysate (ab166833)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- MCF7 whole cell lysate (ab29537)
- Mouse heart normal tissue lysate - total protein (ab30291)
- MCF7 whole cell lysate (ab3871)
- HepG2 whole cell lysate (ab7900)
- HL60 whole cell lysate (ab7914)
- Mouse spleen tissue lysate - total protein (ab7937)
Our Abpromise guarantee covers the use of ab210756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/200. Predicted molecular weight: 43 kDa.|
|IHC-P||1/100 - 1/200.|
FunctionCatalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
PathwayPyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Involvement in diseaseDefects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
Sequence similaritiesBelongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
modificationsThe uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- DHOdehase antibody
- Dhodh antibody
- Dihydroorotate dehydrogenase (quinone) antibody
All lanes : Anti-DHODH antibody (ab210756) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : HL60 cell extract
Lane 3 : SW620 cell extract
Lane 4 : HepG2 cell extract
Lane 5 : Mouse heart extract
Lane 6 : Mouse spleen extract
Lane 7 : Mouse kidney extract
Lane 8 : Rat heart extract
Lane 9 : Rat kidney extract
Predicted band size: 43 kDa
ab210756 has not yet been referenced specifically in any publications.