Overview

  • Product name

  • Description

    Rabbit polyclonal to DHODH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human DHODH aa 280-395 (C terminal).
    Database link: Q02127

  • Positive control

    • MCF7, HL60, SW620 and HepG2 cell extracts; Mouse heart, spleen and kidney extracts; Rat heart and kidney extracts; Human esophagus, colon, gastric and liver cancer tissues; Mouse brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab210756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/200. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/200.

Target

  • Function

    Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
  • Pathway

    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
  • Involvement in disease

    Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
  • Sequence similarities

    Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
  • Post-translational
    modifications

    The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DHOdehase antibody
    • Dhodh antibody
    • Dihydroorotate dehydrogenase (quinone) antibody
    • Dihydroorotate dehydrogenase antibody
    • Dihydroorotate dehydrogenase mitochondrial antibody
    • Dihydroorotate oxidase antibody
    • Human complement of yeast URA1 antibody
    • mitochondrial antibody
    • POADS antibody
    • PYRD_HUMAN antibody
    • URA1 antibody
    see all

Images

  • All lanes : Anti-DHODH antibody (ab210756) at 1/500 dilution

    Lane 1 : MCF7 cell extract
    Lane 2 : HL60 cell extract
    Lane 3 : SW620 cell extract
    Lane 4 : HepG2 cell extract
    Lane 5 : Mouse heart extract
    Lane 6 : Mouse spleen extract
    Lane 7 : Mouse kidney extract
    Lane 8 : Rat heart extract
    Lane 9 : Rat kidney extract

    Predicted band size: 43 kDa

References

ab210756 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (T cells from mouse spleen)
Permeabilization
Yes - True-Nuclear Transcription Factor Buffer Set, Cat: 424401
Gating Strategy
Cell size, singlets, live/dead cells and population-specific markers
Specification
T cells from mouse spleen
Preparation
Cell harvesting/tissue preparation method: Mouse CD4+ T Cells were isolated from the spleen using EasySep™ Isolation Kit (STEMCELL™ technologies)) and stimulated in-vitro using anti-CD3/anti-CD28 prior to analysis.
Sample buffer: PBS, 2% FBS, 1mM EDTA
Fixation
True-Nuclear Transcription Factor Buffer Set, Cat: 424401

Mr. David Ezuz

Verified customer

Submitted Nov 28 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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