Key features and details
- Rabbit polyclonal to DHODH
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-DHODH antibody
See all DHODH primary antibodies
DescriptionRabbit polyclonal to DHODH
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human, Pig
Predicted to work with: Cow, Orangutan
Recombinant fragment (His-T7-tag) corresponding to Human DHODH aa 35-392. Expressed in E.coli. N-terminal tags.
RFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLG HKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVF RLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKN KTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLT KVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNT TVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGG VSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGG VTDAIGAD
Database link: Q02127
- WB: Recombinant human DHODH protein; Rat ovary lysate; Pig liver, spleen and pancreas lysates. IHC-P: Human skin cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 43 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionCatalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
PathwayPyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Involvement in diseaseDefects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
Sequence similaritiesBelongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
modificationsThe uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- DHOdehase antibody
- Dhodh antibody
- Dihydroorotate dehydrogenase (quinone) antibody
All lanes : Anti-DHODH antibody (ab232767) at 2 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : Pig spleen lysate
Lane 3 : Pig pancreas lysate
Lane 4 : Rat ovary lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution
Predicted band size: 43 kDa
Formalin-fixed, paraffin-embedded human skin cancer tissue stained for DHODH using ab232767 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Anti-DHODH antibody (ab232767) at 2 µg/ml + Recombinant human DHODH protein
HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution
Predicted band size: 43 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232767 has been referenced in 1 publication.
- Pesini A et al. Brain pyrimidine nucleotide synthesis and Alzheimer disease. Aging (Albany NY) 11:8433-8462 (2019). PubMed: 31560653