Overview

  • Product name

  • Description

    Rabbit polyclonal to DHODH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human, Pig
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Human DHODH aa 35-392. Expressed in E.coli. N-terminal tags.
    Sequence:

    RFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLG HKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVF RLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKN KTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLT KVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNT TVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGG VSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGG VTDAIGAD


    Database link: Q02127

  • Positive control

    • WB: Recombinant human DHODH protein; Rat ovary lysate; Pig liver, spleen and pancreas lysates. IHC-P: Human skin cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab232767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
  • Pathway

    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
  • Involvement in disease

    Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
  • Sequence similarities

    Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
  • Post-translational
    modifications

    The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DHOdehase antibody
    • Dhodh antibody
    • Dihydroorotate dehydrogenase (quinone) antibody
    • Dihydroorotate dehydrogenase antibody
    • Dihydroorotate dehydrogenase mitochondrial antibody
    • Dihydroorotate oxidase antibody
    • Human complement of yeast URA1 antibody
    • mitochondrial antibody
    • POADS antibody
    • PYRD_HUMAN antibody
    • URA1 antibody
    see all

Images

  • All lanes : Anti-DHODH antibody (ab232767) at 2 µg/ml

    Lane 1 : Pig liver lysate
    Lane 2 : Pig spleen lysate
    Lane 3 : Pig pancreas lysate
    Lane 4 : Rat ovary lysate

    Secondary
    All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution

    Predicted band size: 43 kDa

  • Formalin-fixed, paraffin-embedded human skin cancer tissue stained for DHODH using ab232767 at 20 μg/ml in immunohistochemical analysis. DAB staining.

  • Anti-DHODH antibody (ab232767) at 2 µg/ml + Recombinant human DHODH protein

    Secondary
    HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution

    Predicted band size: 43 kDa

References

ab232767 has not yet been referenced specifically in any publications.

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