Key features and details
- Rabbit polyclonal to DHODH
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DHODH antibody
See all DHODH primary antibodies
DescriptionRabbit polyclonal to DHODH
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Orangutan
- IHC-P: Human kidney, liver, endometrium and duodenum tissue. WB: DHODH over expressed HEK-239T lysate. ICC/IF: MCF7 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 42 kDa.|
FunctionCatalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
PathwayPyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Involvement in diseaseDefects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
Sequence similaritiesBelongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
modificationsThe uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- DHOdehase antibody
- Dhodh antibody
- Dihydroorotate dehydrogenase (quinone) antibody
PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for DHOHD (green) using ab246893 at 4 μg/ml in ICC/IF.
All lanes : Anti-DHODH antibody (ab246893) at 0.4 µg
Lane 1 : Vector only-transfected HEK-293T cell lysate
Lane 2 : DHODH over-expression HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Predicted band size: 42 kDa
Western blot analysis in control (vector only transfected HEK293T lysate) and DHODH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419975).
Formalin-fixed, paraffin-embedded human endometrium tissue stained for DHODH with ab246893 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human kidney tissue stained for DHODH with ab246893 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human duodenum tissue stained for DHODH with ab246893 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver tissue stained for DHODH with ab246893 at a 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246893 has not yet been referenced specifically in any publications.