Anti-DHODH antibody (ab246901)
Key features and details
- Rabbit polyclonal to DHODH
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DHODH antibody
See all DHODH primary antibodies -
Description
Rabbit polyclonal to DHODH -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human DHODH aa 50-200.
Database link: Q02127 -
Positive control
- IHC-P: Human kidney, liver, placenta and colon tissue. WB: MCF7 cell lysate. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab246901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 43 kDa.
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Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 43 kDa. |
Target
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Function
Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. -
Pathway
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. -
Involvement in disease
Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases. -
Sequence similarities
Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily. -
Post-translational
modificationsThe uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 533873 Cow
- Entrez Gene: 1723 Human
- Entrez Gene: 56749 Mouse
- Entrez Gene: 100174634 Orangutan
- Entrez Gene: 65156 Rat
- Omim: 126064 Human
- SwissProt: Q5E9W3 Cow
- SwissProt: Q02127 Human
see all -
Alternative names
- DHOdehase antibody
- Dhodh antibody
- Dihydroorotate dehydrogenase (quinone) antibody
see all
Images
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Anti-DHODH antibody (ab246901) at 0.4 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate
Predicted band size: 43 kDa -
PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for DHODH (green) using ab246901 at 4 µg/ml in ICC/IF.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for DHODH with ab246901 at a 1/20 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for DHODH with ab246901 at a 1/20 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for DHODH with ab246901 at a 1/20 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver tissue stained for DHODH with ab246901 at a 1/20 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab246901 has not yet been referenced specifically in any publications.