Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11814] to DHODH
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-DHODH antibody [EPR11814]
See all DHODH primary antibodies
DescriptionRabbit monoclonal [EPR11814] to DHODH
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human DHODH. The exact sequence is proprietary.
Database link: Q02127
- WB: SKBR-3 cell lysate. Human fetal heart tissue lysate. MCF7, HepG2, Human heart, Mouse heart, Rat heart and Jurkat whole cell lysate (ab7899).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab174288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 43 kDa.|
FunctionCatalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
PathwayPyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Involvement in diseaseDefects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
Sequence similaritiesBelongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
modificationsThe uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- DHOdehase antibody
- Dhodh antibody
- Dihydroorotate dehydrogenase (quinone) antibody
All lanes : Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human heart lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
All lanes : Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution
Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
Note: ab174288 is diluted in 1% BSA.
ab174288 has been referenced in 1 publication.
- Hosseini M et al. UVB-induced DHODH upregulation, which is driven by STAT3, is a promising target for chemoprevention and combination therapy of photocarcinogenesis. Oncogenesis 8:52 (2019). PubMed: 31551419