Overview

  • Product name

    Anti-DHODH antibody [EPR11814]
    See all DHODH primary antibodies
  • Description

    Rabbit monoclonal [EPR11814] to DHODH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human DHODH. The exact sequence is proprietary.
    Database link: Q02127

  • Positive control

    • WB: SKBR-3 cell lysate. Human fetal heart tissue lysate. MCF7, HepG2, Human heart, Mouse heart, Rat heart and Jurkat whole cell lysate (ab7899).
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 43 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
    • Pathway

      Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
    • Involvement in disease

      Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
    • Sequence similarities

      Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
    • Post-translational
      modifications

      The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • DHOdehase antibody
      • Dhodh antibody
      • Dihydroorotate dehydrogenase (quinone) antibody
      • Dihydroorotate dehydrogenase antibody
      • Dihydroorotate dehydrogenase mitochondrial antibody
      • Dihydroorotate oxidase antibody
      • Human complement of yeast URA1 antibody
      • mitochondrial antibody
      • POADS antibody
      • PYRD_HUMAN antibody
      • URA1 antibody
      see all

    Images

    • All lanes : Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution (Purified)

      Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Human heart lysates
      Lane 3 : Mouse heart lysates
      Lane 4 : Rat heart lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 43 kDa
      Observed band size: 43 kDa

    • All lanes : Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution

      Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate
      Lane 2 : Human fetal heart tissue lysate
      Lane 3 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
      Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
      Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa



      Note: ab174288 is diluted in 1% BSA.

    References

    ab174288 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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