Recombinant
RabMAb

Anti-DHODH antibody [EPR11814] (ab174288)

Overview

  • Product name
    Anti-DHODH antibody [EPR11814]
    See all DHODH primary antibodies
  • Description
    Rabbit monoclonal [EPR11814] to DHODH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human DHODH. The exact sequence is proprietary.
    Database link: Q02127

  • Positive control
    • SKBR-3 cell lysate. Human fetal heart tissue lysate. MCF7, HepG2 and Jurkat cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
    • Pathway
      Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
    • Involvement in disease
      Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
    • Sequence similarities
      Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
    • Post-translational
      modifications
      The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
    • Cellular localization
      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • DHOdehase antibody
      • Dhodh antibody
      • Dihydroorotate dehydrogenase (quinone) antibody
      • Dihydroorotate dehydrogenase antibody
      • Dihydroorotate dehydrogenase mitochondrial antibody
      • Dihydroorotate oxidase antibody
      • Human complement of yeast URA1 antibody
      • mitochondrial antibody
      • POADS antibody
      • PYRD_HUMAN antibody
      • URA1 antibody
      see all

    Images

    • All lanes : Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution

      Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate
      Lane 2 : Human fetal heart tissue lysate
      Lane 3 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
      Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
      Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa



      Note: ab174288 is diluted in 1% BSA.

    References

    ab174288 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174288.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up