Recombinant
RabMAb

Recombinant Anti-DHODH antibody [EPR11814] - BSA and Azide free (ab238968)

Overview

  • Product name

    Anti-DHODH antibody [EPR11814] - BSA and Azide free
    See all DHODH primary antibodies
  • Description

    Rabbit monoclonal [EPR11814] to DHODH - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human DHODH. The exact sequence is proprietary.
    Database link: Q02127

  • Positive control

    • WB: Human fetal heart tissue lysate. SK-BR-3, MCF7, HepG2 and Jurkat cell lysates.
  • General notes

    Ab238968 is the carrier-free version of ab174288. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238968 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
    • Pathway

      Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
    • Involvement in disease

      Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
    • Sequence similarities

      Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
    • Post-translational
      modifications

      The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • DHOdehase antibody
      • Dhodh antibody
      • Dihydroorotate dehydrogenase (quinone) antibody
      • Dihydroorotate dehydrogenase antibody
      • Dihydroorotate dehydrogenase mitochondrial antibody
      • Dihydroorotate oxidase antibody
      • Human complement of yeast URA1 antibody
      • mitochondrial antibody
      • POADS antibody
      • PYRD_HUMAN antibody
      • URA1 antibody
      see all

    Images

    • All lanes : Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution

      Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate
      Lane 2 : Human fetal heart tissue lysate
      Lane 3 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
      Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
      Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa



      Note: ab174288 is diluted in 1% BSA.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, tissue culture supernatant and sodium azide (ab174288).

    References

    ab238968 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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