Product nameAnti-DHODH antibody [EPR11814] - BSA and Azide free
See all DHODH primary antibodies
DescriptionRabbit monoclonal [EPR11814] to DHODH - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human DHODH. The exact sequence is proprietary.
Database link: Q02127
- WB: Human fetal heart tissue lysate. SK-BR-3, MCF7, HepG2 and Jurkat cell lysates.
Ab238968 is the carrier-free version of ab174288. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab238968 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab238968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.|
FunctionCatalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
PathwayPyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Involvement in diseaseDefects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
Sequence similaritiesBelongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
modificationsThe uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- DHOdehase antibody
- Dhodh antibody
- Dihydroorotate dehydrogenase (quinone) antibody
All lanes : Anti-DHODH antibody [EPR11814] (ab174288) at 1/1000 dilution
Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
Note: ab174288 is diluted in 1% BSA.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, tissue culture supernatant and sodium azide (ab174288).
ab238968 has not yet been referenced specifically in any publications.