Key features and details
- Rabbit polyclonal to DHPS/DHS
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DHPS/DHS antibody
See all DHPS/DHS primary antibodies
DescriptionRabbit polyclonal to DHPS/DHS
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
- Hela, Jurkat, NIH-3T3, TCMK cell lysates
Protein previously labeled as DHPS.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/500 - 1/2500. Predicted molecular weight: 41 kDa.|
FunctionCatalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.
PathwayProtein modification; eIF5A hypusination.
Sequence similaritiesBelongs to the deoxyhypusine synthase family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Deoxyhypusine synthase antibody
- Dhps antibody
- DHS antibody
All lanes : Anti-DHPS/DHS antibody (ab190266) at 1 µg/ml
Lane 1 : Hela whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK whole cell lysate
Lane 5 : NIH-3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 30 seconds
Immunoprecipitation of Hela whole cell lysates (0.5-1 mg per IP reaction; 20% of IP loaded) labeling DHPS/DHS with ab190266 at 1 µg/ml by western blot. DHPS/DHS was immunoprecipitated using ab190266 (lane 1) or control IgG (lane 2).
ab190266 has been referenced in 2 publications.
- Puleston DJ et al. Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation. Cell Metab 30:352-363.e8 (2019). PubMed: 31130465
- Zhang H et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell 76:110-125.e9 (2019). PubMed: 31474573