Key features and details
- Rabbit polyclonal to DHPS/DHS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DHPS/DHS antibody
See all DHPS/DHS primary antibodies
DescriptionRabbit polyclonal to DHPS/DHS
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- WB: DHPS/DHS overexpressing HEK-393T cell lysate. IHC-P: Human fallopian tube, prostate and colon tissue. ICC/IF: U-2 OS cells.
Protein previously labeled as DHPS.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 36,37,41 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionCatalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.
PathwayProtein modification; eIF5A hypusination.
Sequence similaritiesBelongs to the deoxyhypusine synthase family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Deoxyhypusine synthase antibody
- Dhps antibody
- DHS antibody
Paraffin-embedded human colon tissue stained for DHPS/DHS using ab224134 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for DHPS/DHS using ab224134 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human fallopian tube tissue stained for DHPS/DHS using ab224134 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-DHPS/DHS antibody (ab224134) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : DHPS/DHS overexpressing HEK-393T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Developed using the ECL technique.
Predicted band size: 36,37,41 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for DHPS/DHS (green) using ab224134 at 4 µg/ml in ICC/IF.
ab224134 has not yet been referenced specifically in any publications.