Overview

  • Product name

    Anti-DHRS4L2 antibody [OTI4A10]
  • Description

    Mouse monoclonal [OTI4A10] to DHRS4L2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human DHRS4L2. (NP_932349) produced in E.coli.
    Database link: Q6PKH6

  • Positive control

    • IHC-P: Human testis and placenta tissue. WB: pCMV6-ENTRY DHRS4L2 cDNA transfected HEK-293T cell lysate. HepG2 cell and mouse kidney tissue extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    OTI4A10
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 23 kDa.
IHC-P 1/250.

Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.

Target

  • Relevance

    DHRS4L2 belongs to the short chain dehydrogenases/reductases (SDR) family. The short-chain dehydrogenases/reductases family (SDR) is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent oxidoreductases.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Dehydrogenase/reductase (SDR family) member 4 like 2 antibody
    • Dehydrogenase/reductase (SDR family) member 4 like 2A3 antibody
    • Dehydrogenase/reductase SDR family member 4 like 2 antibody
    • DHRS4L 2 antibody
    • MGC905 antibody
    • NADP(H) dependent retinol dehydrogenase/reductase short isoform like protein antibody
    • SDR25C3 antibody
    • Short chain dehydrogenase/reductase family 25C, member 3 antibody
    see all

Images

  • All lanes : Anti-DHRS4L2 antibody [OTI4A10] (ab236380) at 1/500 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extract
    Lane 2 : Mouse kidney tissue extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 23 kDa

  • Paraffin-embedded human testis tissue stained for DHRS4L2 with ab236380 at a 1/250 dilution in immunohistochemical analysis.

  • Paraffin-embedded human placenta tissue stained for DHRS4L2 with ab236380 at a 1/250 dilution in immunohistochemical analysis.

  • All lanes : Anti-DHRS4L2 antibody [OTI4A10] (ab236380) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY DHRS4L2 cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 23 kDa

References

ab236380 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236380.
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