Anti-DHTKD1 antibody (ab220098)
Key features and details
- Rabbit polyclonal to DHTKD1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DHTKD1 antibody
See all DHTKD1 primary antibodies -
Description
Rabbit polyclonal to DHTKD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment corresponding to Human DHTKD1 aa 1-150.
Database link: Q96HY7 -
Positive control
- Human kidney tissue; RT-4 cell extracts; MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 103 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 103 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). -
Sequence similarities
Belongs to the alpha-ketoglutarate dehydrogenase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 55526 Human
- Entrez Gene: 100173320 Orangutan
- Omim: 614984 Human
- SwissProt: Q96HY7 Human
- SwissProt: Q5R7H0 Orangutan
- Unigene: 104980 Human
- Unigene: 711138 Human
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Alternative names
- AMOXAD antibody
- CMT2Q antibody
- Dehydrogenase E1 and transketolase domain containing 1 antibody
see all
Images
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Anti-DHTKD1 antibody (ab220098) at 1/100 dilution + RT-4 cell extract
Predicted band size: 103 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DHTKD1 with ab220098 at 1/50 dilution.
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Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling DHTKD1 with ab220098 at 4 µg/ml (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab220098 has not yet been referenced specifically in any publications.