Key features and details
- Rabbit polyclonal to DHTKD1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DHTKD1 antibody
See all DHTKD1 primary antibodies
DescriptionRabbit polyclonal to DHTKD1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- Human kidney tissue; RT-4 cell extracts; MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab220098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 103 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionThe 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
Sequence similaritiesBelongs to the alpha-ketoglutarate dehydrogenase family.
- Information by UniProt
- AMOXAD antibody
- CMT2Q antibody
- Dehydrogenase E1 and transketolase domain containing 1 antibody
Anti-DHTKD1 antibody (ab220098) at 1/100 dilution + RT-4 cell extract
Predicted band size: 103 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DHTKD1 with ab220098 at 1/50 dilution.
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling DHTKD1 with ab220098 at 4 µg/ml (green).
ab220098 has not yet been referenced specifically in any publications.